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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 193-201
DOI: https://doi.org/10.20546/ijcmas.2017.605.023


In vitro Activities of Antimicrobial Agents against Uropathogenic Isolates at Brong Ahafo Regional Hospital, Ghana
Solomon Wireko1,2, Amina Abubakari2* and Bempah Opoku2
1Ejisu Government Hospital, Ejisu-Juaben Municipal Assembly-Ghana
2Department of Laboratory Technology, Faculty of Health Sciences, Kumasi Technical University, Kumasi- Ghana
*Corresponding author
Abstract:

Bacterial resistance against antimcrobial agents is a growing international problem in the treatment of nosocomial infections, especially in developing countries. This study therefore sought to determine the antibiotic susceptibility pattern of the isolates in UTIs and to identify the probable antibiotic alternatives in uropathogenic infection to guide antimicrobial therapy at the Brong-Ahafo Regional Hospital-Sunyani. Between January and December 2014, 200 urinary isolates were received from in and out patients at the Microbiology unit of the laboratory. Isolates were tested for antimicrobial susceptibility by the Kirby Bauer disc diffusion method, on Mueller-Hinton Agar (Oxoid GmbH, Wesel, Germany) and the results were presented as resistant or susceptible, according to the recommendations of Clinical and Laboratory Standards Institute (CLSI). One hundred and seventy-two (172, 96.6%) isolates were resistant to tetracycline and 144(90.5%) isolates were resistant to Ampicillin/sulbactam. One hundred and fifty (150, 93.2%) isolates were readily susceptible to Amikacin. Among the third generation cephalosporins, ceftizoxime achieved 50% sensitivity and 20.6% and 16.7% for cefotaxime and ceftazidime respectively against all isolates. The isolates also showed strong resistance to the fluoroquinolones, nalidixic acid (80.8%); ciprofloxacin (74.1%); ofloxacin (65.4%) and levofloxacin (64.6%). Chloramphenicol and gentamicin achieved 23.4% and 24.1% sensitivity respectively. Lower resistance was observed in amikacin and ceftizoxime. The extent of resistance among bacteria isolates in UTIs in non-hospitalized and hospitalized patients is worryingly high in the Brong-Ahafo Regional Hospital. Antimicrobials such as tetracycline, ampicillin/sulbactam, chloramphinicol and gentamicin should no longer be recommended for initial empirical therapies for UTIs especially when E. coli is concerned. Amikacin, ceftizoxime, ofloxacin and levofloxacin may be considered as alternatives.


Keywords: Enterobacteriaceae, In vitro, Antimicrobial, Uropathogenic.
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How to cite this article:

Solomon Wireko, Amina Abubakari and Bempah Opoku. 2017. In vitro Activities of Antimicrobial Agents against Uropathogenic Isolates at Brong Ahafo Regional Hospital, GhanaInt.J.Curr.Microbiol.App.Sci. 6(5): 193-201. doi: https://doi.org/10.20546/ijcmas.2017.605.023