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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(10): 489-500
DOI: https://doi.org/10.20546/ijcmas.2021.1010.059


Biosynthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles by Aspergillus niger Isolated from the Rotten Onion
Dipeshkumar Patel*, Poorti Tamboli and Rupesh Jha
Department of Microbiology, Shri Alpesh N. Patel Post Graduation Institute of Science and Research, Anand, India
*Corresponding author
Abstract:

Present day, researchers turn to natural processes such as using biological microorganisms in order to develop reliable and ecofriendly methods for the synthesis of metallic nanoparticles. In this research, we had investigated extracellular biosynthesis of silver nanoparticles using A. niger, isolated from the rotten onion. The formation of silver nanoparticles in the cell filtrates was confirmed by change in the color of filtrates of cell, absorption peak between 300-600 nm in UV-VIS spectra. There was a logical relationship in the efficiencies of studied A. niger in the production of silver nanoparticles and their nitrate reductase activity. The microscopic analysis of the colonies of A. niger was done by using 0.1% congo red dye. It effectively showed the morphological structure of the fungus with Conidia and Conidiophores. Hence, 0.1% congo red dye was used to stain the fungal smear and study it under the microscope. The present study showed that A. niger have potential for the biosynthesis of silver nanoparticles depending on their nitrate reductase activity.


Keywords: Rotten onion,niger, silver nanoparticles, congo red dye,UV-VIS spectrophotometer
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How to cite this article:

Dipeshkumar Patel, Poorti Tamboli and Rupesh Jha. 2021. Biosynthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles by Aspergillus niger Isolated from the Rotten Onion.Int.J.Curr.Microbiol.App.Sci. 10(10): 489-500. doi: https://doi.org/10.20546/ijcmas.2021.1010.059