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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 129-137
DOI: https://doi.org/10.20546/ijcmas.2017.605.016


Comparative Study of Phytochemical Analysis, Antimicrobial and Antioxidant Activity of Different Root Extracts of Desmostachya bipinnata Stapf (Kush)
K.B. Joshi*, M.K. Mandavia and B.A. Golakiya
Department of Biochemistry and Biotechnology, Junagadh Agricultural University, Junagadh, India
*Corresponding author
Abstract:

The present study was designed to evaluate Preliminary phytochemical analysis, in vitro antimicrobial activity and antioxidant activity of different six extracts of Desmostachya Bipinnata Stapf (Kush). Plant roots were extracted in different six solvents viz. Hexane, ethyl acetate, acetone, Methanol, Water and Methanol: Water (90:10) through Soxtherm according to polarity gradients. The phenolics, flavonoid, tannin and other phytochemicals of the extract were also determined using standard phytochemical reaction methods. Methanol: water and methanol extracts showed the presence of Phenol and glycosides, while in acetone extract terpenoids and glycosides were found. Aiming to investigate antimicrobial activities, agar well diffusion method was followed using three pathogenic bacteria and two fungi as test organisms. The plant root extracts showed moderate antibacterial activities (zone of inhibition (ZOI): 6-9mm) which was compared with standard kanamycin, while extracts showed positive antifungal activities (ZOI: 6-12 mm) and fluconazole was used as standard antifungal agent. We assessed the antioxidant potential of all six extracts of Desmostachya bipinnata Stapf (Kush) using test involving inhibition of DPPH activities. The highest antioxidant activity of acetone extract was noticed at IC50 (Inhibition concentration at 50%) of 17.42μg /ml followed by methanol extract at IC50 of 25.83 μg /ml compared to those of ascorbic acid (7.5 μg /ml). Current studies indicated that plant root extracts possessed moderate antimicrobial activities and good antioxidant activity. So our findings revealed that the acetone extract of Desmostachya bipinnata Stapf (Kush) possess antioxidant properties and could serve as free radical inhibitors or scavenger or, acting possibly as natural antioxidants.


Keywords: Desmostachya bipinnata Stapf, Antimicrobial activity, Antioxidant activity, DPPH
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How to cite this article:

Joshi, K.B., M.K. Mandavia and Golakiya, B.A. 2017. Comparative Study of Phytochemical Analysis, Antimicrobial and Antioxidant Activity of Different Root Extracts of Desmostachya bipinnata Stapf (Kush).Int.J.Curr.Microbiol.App.Sci. 6(5): 129-137. doi: https://doi.org/10.20546/ijcmas.2017.605.016