International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:10, October, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(10): 221-228

Identification and Characterization of Escherichia coli from Bronchial Plug of Broiler Chicken with Respiratory Distress
M. H. Chhatrola1, D. T. Fefar1, A. R. Bhadaniya1*, B. J. Trangadia1,V. A. Kalaria1, S. N. Ghodasara2, B. B. Javiaand J. M. Chauhan1
1Department of Veterinary Pathology,
2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, Gujarat - 362001, India
*Corresponding author

A Twenty-five bronchial plug samples from dead broiler chicken with signs of respiratory distress collected for identification of avian pathogenic E. coli (APEC). Samples comprised of one pooled sample each from 25 poultry farms located in and around Junagadh city. Isolation, culture and colony characteristics used for primary detection followed by PCR assay targeting four virulence genes (iss, papC, tsh and vat)for confirmatory diagnosis. All bronchial plugs were found to be positive for E. coli infection by isolation. Out of 25 flock sampled, the highest number of E. coli isolates were found positive for the presence of iss gene (22) followed by tsh gene (15), vat gene (13) and papC gene (8)by PCR. On antibiogram, overall E. coli was highly sensitive to colistin (Methane sulphonate) and meropenem(100%) followed by levofloxacin (76%), ceftriaxone (64%), gentamicin (60%), amikacin (60%), co-trimoxazole (40%), amoxycilin-clavunic acid (8%) and imepenem (8%). These results indicated the high prevalence of the E. coli with variable antibiotic resistance in broiler chicken.

Keywords: Poultry, E coli, Antibiotics, Respiratory distress

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How to cite this article:

Chhatrola, M. H., D. T. Fefar, A. R. Bhadaniya, B. J. Trangadia, V. A. Kalaria, S. N. Ghodasara, B. B. Javia and Chauhan, J. M. 2021. Identification and Characterization of Escherichia coli from Bronchial Plug of Broiler Chicken with Respiratory Distress.Int.J.Curr.Microbiol.App.Sci. 10(10): 221-228. doi:
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