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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(6): 532-538
DOI: https://doi.org/10.20546/ijcmas.2021.1006.058


Comparative Evaluation of Gene Xpert and Various Staining Techniques in the Diagnosis of Pulmonary Tuberculosis
Loveena Oberoi1, Naveen Pandhi2, Muskan Khullar1* and Tavishi Oberoi1
1Department of Microbiology, 2Department of Chest and TB, Government Medical College, Amritsar, India
*Corresponding author
Abstract:

Tuberculosis (TB) is an ancient communicable disease caused by Mycobacterium tuberculosis. In India, RNTCP (Revised national tuberculosis control program) now known as NTEP (National tuberculosis elimination program) endorsed diagnostic steps to diagnose TB infection and disease are followed. To study the diagnostic efficacy of GeneXpert in comparison to various staining techniques in patients suspected of pulmonary tuberculosis. 150 sputum samples from patients clinically suspected of pulmonary tuberculosis were processed and subjected to Ziehl-Neelsen staining, Kinyoun Staining and Fluorescent staining. Simultaneously GeneXpert was performed on unprocessed samples. Out of 150 samples, GeneXpert detected 46 (30.67%) cases followed by Fluorescent staining, 44(29.33%), ZN staining 43 (28.67%) cases and Kinyoun staining detected the least i.e. 35(23.33%). Amongst 46 positive cases detected by GeneXpert 7(15.22%) cases were found to be resistant to Rifampicin. Hence it proved the efficacy and sensitivity of GeneXpert over staining methods. Tuberculosis is a global pandemic. Its early diagnosis and timely initiation of treatment is necessary. Therefore, GeneXpert when used in conjunction with microscopy improves detection of tuberculosis and multi drug resistant tuberculosis.


Keywords: Ziehl Neelsen (ZN) staining, Kinyoun staining, Fluorescent staining, Tuberculosis (TB), Mycobacterium tuberculosis (MTB), N-acetyl-L-cysteine (NALC)
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How to cite this article:

Loveena Oberoi, Naveen Pandhi, Muskan Khullar and Tavishi Oberoi. 2021. Comparative Evaluation of Gene Xpert and Various Staining Techniques in the Diagnosis of Pulmonary Tuberculosis.Int.J.Curr.Microbiol.App.Sci. 10(6): 532-538. doi: https://doi.org/10.20546/ijcmas.2021.1006.058