International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:6, June, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(6): 513-518

Extraction and Characterization of Chitosan by Simple Technique from Mud Crabs
A. Musmade* and Lalit Mahatma
Department of Plant Pathology, N.M. College of Agriculture, Navsari Agricultural University, Navsari- 396 450, Gujarat, India
*Corresponding author

Chitosan is a naturally available biopolymer. It occurs as a component of crustacean shells, insect exoskeletons, fungal cell walls and plankton. In this work, chitin was extracted from mud crab shells waste. Chitosan, a useful biopolymer, was obtained from pulverized shells of crabs via demineralization, deproteination and de-acetylation. These steps were crucial for the elimination of calcium carbonate and other minerals as well as protein which are present in the shells. In this study, the chemical compositions of crab shells waste were analyzed by Fourier transform infrared spectroscopy (FTIR). From the 200 g exoskeleton of mud crab, 52.46 g (26.23%) chitin was extracted. Out of the 52.46 g chitin, 38.68 g (73.73%) chitosan was obtained.

Keywords: Chitin, chitosan, FTIR, Deacetylation, Mud Crab

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How to cite this article:

Musmade, N. A. and Lalit Mahatma. 2021. Extraction and Characterization of Chitosan by Simple Technique from Mud Crabs.Int.J.Curr.Microbiol.App.Sci. 10(6): 513-518. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.