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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(5): 429-435
DOI: https://doi.org/10.20546/ijcmas.2021.1005.050


Stability and Specific Substrate of Lipase from Microbes  in Coconut Pulp Media
Dewa Gde Mayun Permana* and Ni Luh Ari Yusasrini
Food Science and Technology Study Program, Faculty of Agricultural Technology,
Udayana University, Bukit Jimbaran Badung, Bali, Indonesia
*Corresponding author
Abstract:

One source of lipase that is considered potential is from microbes because microbes can multiply rapidly. The advantage of microbes is being able to adapt quickly to the environment, including growing media. Coconut pulp still contains fat with lauric acid as the dominant fatty acid. The purpose of this study was to obtain temperature and pH stability and lipase specific substrate from microbes grown on coconut pulp media. To achieve this goal a partial purification of lipase was carried out with ammonium sulfate. The obtained lipases were tested for temperature and pH stability by hydrolysis activity. Specific substrates were determined by testing esterification activity using several types of fatty acids. The results showed that at the saturation level of 60% ammonium sulfate produced lipase with the highest level of activity. The resulting lipase has a stability of more than 90% at 40°C and pH 6 is the most stable activity. Substrates that show high activity are lauric acid and oleic acid.


Keywords: Stability, Specific substrate, lipase, microbe,coconut pulp
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How to cite this article:

Dewa Gde Mayun Permana, I. and Ni Luh Ari Yusasrini. 2021. Stability and Specific Substrate of Lipase from Microbes in Coconut Pulp Media.Int.J.Curr.Microbiol.App.Sci. 10(5): 429-435. doi: https://doi.org/10.20546/ijcmas.2021.1005.050