National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
One source of lipase that is considered potential is from microbes because microbes can multiply rapidly. The advantage of microbes is being able to adapt quickly to the environment, including growing media. Coconut pulp still contains fat with lauric acid as the dominant fatty acid. The purpose of this study was to obtain temperature and pH stability and lipase specific substrate from microbes grown on coconut pulp media. To achieve this goal a partial purification of lipase was carried out with ammonium sulfate. The obtained lipases were tested for temperature and pH stability by hydrolysis activity. Specific substrates were determined by testing esterification activity using several types of fatty acids. The results showed that at the saturation level of 60% ammonium sulfate produced lipase with the highest level of activity. The resulting lipase has a stability of more than 90% at 40°C and pH 6 is the most stable activity. Substrates that show high activity are lauric acid and oleic acid.
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