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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(5): 294-305
DOI: https://doi.org/10.20546/ijcmas.2021.1005.037


Isolation and Identification of Pathogenic Bacterial Contaminants associated with Poultry
Rujuta Kedar Karkhanis*, Yashsvi Udaybhai Raval,Devikaben Bharatbhai Vishani and Dhruv Girishbhai Patel
Department of Microbiology, Parul Institute of Applied Science, Waghodia, India
*Corresponding author
Abstract:

The study was conducted aiming at the isolation and identification of Salmonella and Escherichia coli (E. coli) from different poultry farm in Padra Village, Gujarat, India. Drinking water samples were collected from feeding operation of two different poultry farms and were analysed for the presence of Salmonella spp. and Escherichia coli using microbiological analysis. The samples were analysed by culturing in different media such as nutrient broth (NB), nutrient Agar (NA), BGA (brilliant-green Agar), MCA (MacConkey), EMB (eosin methylene blue), and LuriaBertani media. Salmonella spp. and Escherichia coli were tested against 5 anti- microbial agents: Gentamicin, Streptomycin, Ampicillin, Penicillin, Chloramphenicol using the Kirby-Bauer disc diffusion method. The result of this research showed the presence of aerobic bacteria and also the resistant of bacteria to certain antibiotics in water samples. Strict hygienic practices and biosecurity measures should be instituted and maintained both at feed mills and poultry farms so as to eliminate contamination associated with poultry. The widespread occurrence of Salmonella spp. and E. coli in poultry feeds reinforces the need for effective control measures, hygiene in processing and handling of feeds.


Keywords: Salmonella spp., Escherichia coli, poultry farm, feeding operation, microbiological analysis
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How to cite this article:

Rujuta Kedar Karkhanis, Yashsvi Udaybhai Raval, Devikaben Bharatbhai Vishani and Dhruv Girishbhai Patel. 2021. Isolation and Identification of Pathogenic Bacterial Contaminants associated with Poultry.Int.J.Curr.Microbiol.App.Sci. 10(5): 294-305. doi: https://doi.org/10.20546/ijcmas.2021.1005.037