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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(5): 112-126
DOI: https://doi.org/10.20546/ijcmas.2021.1005.016


Isolation and Biochemical Analysis of Bacteria associated with Dried Stigma of Saffron (Crocus sativus)
Mridhu Sharma*, Puneet Gupta, Shanu Mangotra, Sneha Ganjoo, Deepika Trakroo, Sakshi Sharma and Jyoti Vakhlu
Department of Biotechnology, University of Jammu, School of Biotechnology, Baba Saheb Ambedkar Road, Jammu, Tawi (J&K) -180006, India
*Corresponding author
Abstract:

The stigma and pistil of saffron are used to extract chemical compounds such as crocin, picrocrocin, crocetin and safranal; and volatile oils for flavor respectively. The work is to isolate microbiome associated with stigma of saffron. 15 morphotypes obtained from Kashmir and 32 from Kishtwar. Their morphological characterization showed that the smooth textured colonies more in Kishtwar and rough textured colonies more in Kashmir. The IC50 values of radical scavenging revealed that antioxidant activity of Kishtwar is more as lower IC50 value corresponds to a higher antioxidant activity of sample. The IC50 values of ferrous chelation revealed that antioxidant activity of Kishtwar is more as lower IC50 value corresponds to a higher antioxidant activity of sample. Hence, Kishtwar has more antioxidant activity. The flavonoid and phenolic content of Kashmir is higher than that of Kishtwar. Of the biochemical assays conducted, only tannins and Glycosides are present in stigma of saffron. Further work can be done to unveil the bacterial property to produce the pigments that act as favouring agent that can be used in bio-industry.


Keywords: Microbiome, plants, temperature, moisture and pH
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How to cite this article:

Mridhu Sharma, Puneet Gupta, Shanu Mangotra, Sneha Ganjoo, Deepika Trakroo, Sakshi Sharma and Jyoti Vakhlu. 2021. Isolation and Biochemical Analysis of Bacteria associated with Dried Stigma of Saffron (Crocus sativus).Int.J.Curr.Microbiol.App.Sci. 10(5): 112-126. doi: https://doi.org/10.20546/ijcmas.2021.1005.016