National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Insect bioassays were carried out of field strain of Helicoverpa armigera that survived on Bt cotton. These insects were reared on Cry1Ac incorporated diet that served as the initial culture for selection of resistance. Based on the partial sequences of putative trypsin mRNA of Helicoverpa armigera (accession no. AFO45138) trypsin specific primers were designed. A part of trypsin specific gene that includes the catalytic site (ASP102 and Ser195) was sequenced in Cry1Ac susceptible and resistant strains of Helicoverpa armigera. Sequencing demonstrates four amino acid changes in susceptible and resistant strains. Threonine, aspartic acid, serine and histidine at 180, 185, 186 and 192 amino acid positions were substituted with alanine, valine, glycine and glutimic acid respectively in resistant strain.
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