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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(5): 79-85
DOI: https://doi.org/10.20546/ijcmas.2021.1005.012


Partial Sequencing of Putative Trypsin cDNAs in Cry1Ac Susceptible and Resistant Strains of Helicoverpa armigera (Hubner)
K. M. Sharma*, A. A. Bharose and R. N. Dhawale
Vilasrao Deshmukh College of Agricultural Biotechnology, Latur, VNMKV, Parbhani, India
*Corresponding author
Abstract:

Insect bioassays were carried out of field strain of Helicoverpa armigera that survived on Bt cotton. These insects were reared on Cry1Ac incorporated diet that served as the initial culture for selection of resistance. Based on the partial sequences of putative trypsin mRNA of Helicoverpa armigera (accession no. AFO45138) trypsin specific primers were designed. A part of trypsin specific gene that includes the catalytic site (ASP102 and Ser195) was sequenced in Cry1Ac susceptible and resistant strains of Helicoverpa armigera. Sequencing demonstrates four amino acid changes in susceptible and resistant strains. Threonine, aspartic acid, serine and histidine at 180, 185, 186 and 192 amino acid positions were substituted with alanine, valine, glycine and glutimic acid respectively in resistant strain.


Keywords: Bacillus thuringiensis, Helicoverpa armigera, Cry1Ac resistant, Cry1Ac susceptible, trypsin cDNAs
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How to cite this article:

Sharma, K. M., A. A. Bharose and Dhawale, R. N. 2021. Partial Sequencing of Putative Trypsin cDNAs in Cry1Ac Susceptible and Resistant Strains of Helicoverpa armigera (Hubner).Int.J.Curr.Microbiol.App.Sci. 10(5): 79-85. doi: https://doi.org/10.20546/ijcmas.2021.1005.012