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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 2523-2532
DOI: https://doi.org/10.20546/ijcmas.2017.604.294


Rapid Detection of Salmonella spp. in Animal Origin Foods by In-House Developed Loop-Mediated Isothermal Amplification (LAMP) Assay
V.R. Masoji, R.J. Zende, R.D. Suryawanshi, V.M. Vaidya, R.N. Waghamare,D.P. Kshirsagar*, R.P. Todankar and A.H. Shirke
Department of Veterinary Public Health and Epidemiology,Bombay Veterinary College, Parel, Mumbai, India
*Corresponding author
Abstract:

Salmonellosis is one of the most common causal food borne disease in India. An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella spp. isolates in foods of animal origin from Mumbai, India. The aim of this study was to develop in-house LAMP for simple and inexpensive detection of Salmonella spp. in animal origin foods using specifically designed primers targeting invA gene contains sequences unique to this genus. The reaction was optimized using genomic DNA of S. typhimurium (MTCC 3224) as the template. The assay was conducted in a water bath for 1h at 65°C. The results were visualized after addition of SYBR Green® fluorescent dye. The test was further evaluated on 59 serotyped Salmonella field isolates to ensure its reliability and usefulness. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and 10 times more sensitive in detecting Salmonella spp. compared to the optimized conventional polymerase chain reaction (PCR) method. This simple method may be applied on field diagnostic laboratories without access to expensive equipment.


Keywords: Loop-mediated isothermal amplification (LAMP), Salmonella spp., Polymerase Chain Reaction (PCR), Animal origin foods
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How to cite this article:

Masoji, V.R., R.J. Zende, R.D. Suryawanshi, V.M. Vaidya, R.N. Waghamare, D.P. Kshirsagar, R.P. Todankar and Shirke, A.H. 2017. Rapid Detection of Salmonella spp. in Animal Origin Foods by In-House Developed Loop-Mediated Isothermal Amplification (LAMP) Assay.Int.J.Curr.Microbiol.App.Sci. 6(4): 2523-2532. doi: https://doi.org/10.20546/ijcmas.2017.604.294