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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(4): 268-286
DOI: https://doi.org/10.20546/ijcmas.2021.1004.028


Identification of Superior Allele for Drought Responsive Myb Gene in different Germplasms of Eleusine coracana through Morpho-Physiological, Biochemical and Sequence Diversity Analysis
Akash Sinha, Megha Bhatt, Ankita Chauhan and Pushpa Lohani*
Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, India
*Corresponding author
Abstract:

Abiotic stresses like drought, salinity and cold cause huge losses to crop yield. There is an urgent need to produce drought tolerance in crops in order to meet challenges posed by changing climatic conditions and food security. One of the way in which the challenge could be met is by fishing out superior allele of a drought responsive gene and its utilization in producing drought tolerance in crops. The present study was carried out with the objective to study the variation in morpho-physiological and biochemical parameters of 11 day drought stress treatment on different genotypes of finger millet at the vegetative stage and determine the most drought tolerant genotype. Myb gene sequence of the tolerant genotype was considered as superior allele for drought tolerance. Therefore, DNA was isolated from 12 genotypes of E. coracana and PCR amplification was performed using gene specific primers. The amplicons were sequenced and subjected to further analysis using various bioinformatics tools. Multiple Sequence Analysis results indicated that considerable nucleotide diversity was present in the drought responsive Myb gene sequence in the finger millet germplasm. PRM-6107 was found to be the most drought tolerant genotype, amongst all the genotypes under study based on the various morpho-physiological and biochemical tests. PCPGR-8115 was observed as the most sensitive genotype. Myb gene sequence present in PRM-6107 was considered as superior myb allele. Single nucleotide polymorphisms and insertions-deletions were observed in the myb allele of PRM-6107.


Keywords: Allele, Drought tolerance, morho-physiological, biochemical, sequence diversity, myb gene, SNP
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How to cite this article:

Akash Sinha, Megha Bhatt, Ankita Chauhan and Pushpa Lohani. 2021. Identification of Superior Allele for Drought Responsive Myb Gene in different Germplasms of Eleusine coracana through Morpho-Physiological, Biochemical and Sequence Diversity Analysis.Int.J.Curr.Microbiol.App.Sci. 10(4): 268-286. doi: https://doi.org/10.20546/ijcmas.2021.1004.028