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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 345-353
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.041


 Screening of Tyrosinase Producing Soil Actinomycetes from Shirala Region of Maharashtra, India
Gare Sandip Subhash1*, D.D. Karad2 and S.W. Kulkarni3
1Department of Microbiology, Vishwasrao Naik Art’s, Commerce and Baba Naik Science  Mahavidyalaya, Shirala 415408, Dist-Sangli M.S, India
2Departments of Microbiology and Research Center, Shri Shivaji Mahavidyalaya, Barshi-413 411, Dist-Solapur, MS, India
3Research Department of Microbiology, Shriman Bhausaheb Zadbuke Mahavidyalaya, Barshi 413 401, Dist-Solapur M.S, India
*Corresponding author
Abstract:

A total seven soil samples were collected from the Shirala region Dist-Sangli. The total 70 Actinomycete isolates were obtained from soil by performing serial dilution technique and using Glycerol asparagine agar supplemented with Cycloheximide (100µg/ml). Actinomycete isolates were primarily identified to genus level on the basis of their microscopic and cultural characteristics. Biochemical and other characters were used to identify isolates to species level. A total 70 Actinomycete isolates were identified by using modified DOS based MICRO-IS software such as PIBWin. Out of 70 Actinomycete isolates total 82.85% isolates were belonged to genus Streptomyces, 10% isolates were belonged to genus Streptoverticillum, 2.8571% isolates were belonged to Nocardia, 2.8571% isolates were belonged to genus Micromonospora and rest of the 1.4285% isolate were belonged to Actinomadura. The primary screening of 70 isolates for tyrosinase production were carried out and isolates were showed positive tyrosinase activity.


Keywords: Actinomycetes, Genus, Streptomyces, Tyrosinase, Screening.
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How to cite this article:

Gare Sandip Subhash, Karad, D.D.  and Kulkarni, S.W. 2016. Screening of Tyrosinase Producing Soil Actinomycetes from Shirala Region of Maharashtra, India.Int.J.Curr.Microbiol.App.Sci. 5(3): 345-353. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.041