International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:3, March, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1748-1759

Molecular Characterization of Cotton (Gossypium hirsutum L.) Genotypes Using SSR Markers for Fiber Quality Traits
K. Sarode1*, K. M. Sharma1, N. A. Shinde1, A. R. Gaikwad2,L. Chavhan1 and P. A. Pimpale1
 1Vilasrao Deshmukh College of Agricultural Biotechnology, Latur, (M.S.),
(Under Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani), India
2Cotton Research Station, VNMKV, Nanded, MS, India
*Corresponding author

To assess the genetic diversity and relationships among 25 cotton (Gossypium hirsutum L.) genotypes were analyzed using morphological and molecular markers. Morphological analysis was performed based on 3 fiber quality traits including fiber length, strength and fineness the mean values of each trait for each genotype were calculated. The results showed that most of the genotypes have long fiber length, very high fiber strength and average fiber fineness. The molecular profiling of 25 cotton genotypes were carried out by using 42 SSR markers reported to be linked to quantitative trait loci (QTLs) for fiber quality traits (Fiber length, Fiber strength and Micronaire). Twenty five SSR markers related with fiber quality traits ware found to be polymorphic produced a total of 53 amplicon with an average of 2.12 alleles per locus ranging from 130 bp to 370 bp products, Markers showing 100% polymorphism with average polymorphism information content (PIC) value of 0.290. The BNL-1059 (PIC-0.481), CIR-244 (PIC-0.538), CIR-354 (PIC-0.660), CIR-381 (PIC-0.784), CIR-246 (PIC-0.749) and JESPR153 (PIC-0.639) marker were found to be most informative which indicates significantly diversity between all genotypes for fiber quality traits. Similarity matrix was used to depict dendrogram through UPGMA cluster analysis based on SSR similarity coefficient ranged from 0.61 to 0.91 indicating the fact that large proportions of genotype were dissimilar. The present study revealed that genotypes BN-1, PH-348, and ARB-908 for fiber quality were found to be the most diverse having a broad genetic base for the concerned fiber traits. 

Keywords: Gossipium hirsutum, Fiber quality traits, SSR, Polymorphism, Dendrogram

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How to cite this article:

Sarode, D. K., K. M. Sharma, N. A. Shinde, A. R. Gaikwad, R. L. Chavhan and Pimpale, P. A. 2021. Molecular Characterization of Cotton (Gossypium hirsutum L.) Genotypes Using SSR Markers for Fiber Quality Traits.Int.J.Curr.Microbiol.App.Sci. 10(3): 1748-1759. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.