National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An efficient protocol for organogenesis and plant regeneration has been developed for Dianthus caryophyllus L. cv. ‘Master’ using nodal explant. Optimal callus formation (79.17%) from nodal explants was obtained on MS medium supplemented with 1.5 mg/l 2,4-D and 1.0 mg/l NAA. The highest shoot induction response (65.28%) was recorded on MS medium supplemented with 1.5 mg/l TDZ, 0.25 mg/l Kinetin and 0.25 mg/l NAA leading to maximum average no. of shoots per callus clump (5.14) and shoot length (1.43 cm). The highest percentage of root regeneration (84.44%) was observed on MS medium supplemented with 1.0 mg/l IBA and 100 mg/l activated charcoal. The in vitro raised plantlets were successfully acclimatized under ex vitro conditions and grew well in the glasshouse. All the regenerated plantlets appeared normal with respect to morphological characteristics with 100% survival rate. Somaclonal variation in tissue culture is a common phenomenon which makes it mandatory to check for genetic stability of plants. The genetic variation in the in vitro raised plants was confirmed using Inter Simple Sequence Repeats(ISSR) markers. Comparison of the bands with the mother plant revealed that the genetic variation frequency reached 6.54 % using fifteen ISSR markers.
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