National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Streptococcus suis serotype 2 infection is considered as a major pathogenic disease worldwide both in humans and swine. In the present study, isolation of lytic phages against S. suis serotype 2 would provide therapeutic potential in combating the antibiotic resistant strains of S. suis organisms. Four lytic bacteriophages SS1, SS2, SS3 and SS4 were recovered from the sewage samples at piggery farm. Upon host range determination, SS1, SS2, SS3 phages were found to be lytic against S. suis serotypes 1/2, 2, 3, 7 and 9 where as SS4 phage was found to be lytic against only serotype 2 and 7. SS1, SS2 and SS3 phages were found to be lytic against other gram positive organisms like Methicillin Resistant Staphylococcus aureus, Streptococcus pyogenes, Streptococcus dysgalactiae and Enterococcus sp. indicating broader lytic spectrum. Three different phage morphotypes were observed against S. suis. SS2 phage was having the larger plaque size of 4mm in diameter in comparison to other phages with highest mean phage titre of 2×1014PFU/ml i.e., 14 log PFU count and hence SS2 phage was subjected for temperature, pH and chemical stability testing. SS2 Phage was found to be stable on incubation for 30min at 4oC and 20o C, showing highest activity at 4oC with count of 2×1014 PFU/ml. Phage titre was dropped to 4×104 PFU/ml at 60 oC. Upon pH stability, the SS2 phage was having stable phage counts at pH 2, 7 and 11 whereas highest lytic activity was observed at pH 7 with counts of 2×1015 PFU/ml. Our study provides detailed analysis of isolated S. suis lytic bacteriophage and its partial characterization, which could be a natural therapeutic strategy against antimicrobial resistant S. suis strains.
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