International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:3, March, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1300-1306

Isolation and Partial Characterisation of Streptococcus suis Bacteriophage
Lahari Laddika1, Shumaila Malik1, Renu Chauhan1, Salauddin Qureshi1*,Monalisa Sahoo2, Sagar Patel2 and A. K. Tiwari1
1Division of Biological Standardization, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, India
2Division of Pathology, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, India
*Corresponding author

Streptococcus suis serotype 2 infection is considered as a major pathogenic disease worldwide both in humans and swine. In the present study, isolation of lytic phages against S. suis serotype 2 would provide therapeutic potential in combating the antibiotic resistant strains of S. suis organisms. Four lytic bacteriophages SS1, SS2, SS3 and SS4 were recovered from the sewage samples at piggery farm. Upon host range determination, SS1, SS2, SS3 phages were found to be lytic against S. suis serotypes 1/2, 2, 3, 7 and 9 where as SS4 phage was found to be lytic against only serotype 2 and 7. SS1, SS2 and SS3 phages were found to be lytic against other gram positive organisms like Methicillin Resistant Staphylococcus aureus, Streptococcus pyogenes, Streptococcus dysgalactiae and Enterococcus sp. indicating broader lytic spectrum. Three different phage morphotypes were observed against S. suis. SS2 phage was having the larger plaque size of 4mm in diameter in comparison to other phages with highest mean phage titre of 2×1014PFU/ml i.e., 14 log PFU count and hence SS2 phage was subjected for temperature, pH and chemical stability testing. SS2 Phage was found to be stable on incubation for 30min at 4oC and 20o C, showing highest activity at 4oC with count of 2×1014 PFU/ml. Phage titre was dropped to 4×104 PFU/ml at 60 oC. Upon pH stability, the SS2 phage was having stable phage counts at pH 2, 7 and 11 whereas highest lytic activity was observed at pH 7 with counts of 2×1015 PFU/ml. Our study provides detailed analysis of isolated S. suis lytic bacteriophage and its partial characterization, which could be a natural therapeutic strategy against antimicrobial resistant S. suis strains.

Keywords: Streptococcus suis, Bacteriophage, Antibiotic resistance, PFU

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How to cite this article:

Lahari Laddika, Shumaila Malik, Renu Chauhan, Salauddin Qureshi, Monalisa Sahoo, Sagar Patel and Tiwari, A. K. 2021. Isolation and Partial Characterisation of Streptococcus suis Bacteriophage.Int.J.Curr.Microbiol.App.Sci. 10(3): 1300-1306. doi:
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