International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:3, March, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1246-1256

Standardization of in vitro Regeneration Technique in Elephant Foot Yam (Amorphophallus paeoniifolius L.)
Pravin B. Pawar1*, S. V. Sawardekar1, R. S. Deshpande1, M. G. Palshetkarand R. G. Khandekar3
1Department of Plant Biotechnology, 2Department of Agril. Botany,
3Department of Horticulture, College of Agriculture, Dapoli, Dr. B. S. Konkan Krishi Vidyapeeth, Dapoli – 415 712, Ratnagiri District, M.S., India
*Corresponding author

The present investigation was carried out to standardize surface sterilization and culture establishment technique for in vitro regeneration of elephant foot yam. The well-developed apical buds from the corms of variety Gajendra were used as explant. Among various surface sterilization treatments tried, the treatment T5 i.e., 750 mg/L Carbendazim (overnight) before cut, 12.5 ml/L Sodium hypochlorite (20 min), 10 ml/L Tween 20 (15 min), 10 ml/L Dettol and 50 ml/L Savlon (30 min), 1 g/L Carbendazim (10 min), under running warm water (1 hr), 2 g/L Carbendazim (15 min), 70 % Ethanol (2 min), 0.12 g/L Mercuric Chloride (15 min), 50 ml/L Sodium hypochlorite (5 min), 16 ml/L GA3 (10 min), + 3 ml/L Streptomycin (20 min), 750 mg/L Cefotaxime (I) (20 min) and 750 mg/L Cefotaxime (II) (40 min) was found best combination for achieving the highest percentage of aseptic cultures (88.57%) while, the treatment T2 recorded lowest (20%) establishment of aseptic culture. Among the various combinations, MS medium supplemented with 6 mg/L BAP + 0.5 mg/L NAA showed highest percentage of culture establishment (60%). The media combination of MS + 6 mg/L BAP + 0.5 mg/L NAA + 0.1 g/L Ascorbic acid recorded the highest percentage (88.89%) of shoot induction. Also, it showed minimum (15.33) days for shoot induction. The same media combination found effective for the highest average number of multiple shoot bud induction (5.33).

Keywords: Elephant foot yam, Surface sterilization, Micropropagation, in vitro techniques, Culture establishment

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How to cite this article:

Pravin B. Pawar, S. V. Sawardekar, R. S. Deshpande, M. G. Palshetkar and Khandekar, R. G. 2021. Standardization of in vitro Regeneration Technique in Elephant Foot Yam (Amorphophallus paeoniifolius L.).Int.J.Curr.Microbiol.App.Sci. 10(3): 1246-1256. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.