National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was carried out to standardize surface sterilization and culture establishment technique for in vitro regeneration of elephant foot yam. The well-developed apical buds from the corms of variety Gajendra were used as explant. Among various surface sterilization treatments tried, the treatment T5 i.e., 750 mg/L Carbendazim (overnight) before cut, 12.5 ml/L Sodium hypochlorite (20 min), 10 ml/L Tween 20 (15 min), 10 ml/L Dettol and 50 ml/L Savlon (30 min), 1 g/L Carbendazim (10 min), under running warm water (1 hr), 2 g/L Carbendazim (15 min), 70 % Ethanol (2 min), 0.12 g/L Mercuric Chloride (15 min), 50 ml/L Sodium hypochlorite (5 min), 16 ml/L GA3 (10 min), + 3 ml/L Streptomycin (20 min), 750 mg/L Cefotaxime (I) (20 min) and 750 mg/L Cefotaxime (II) (40 min) was found best combination for achieving the highest percentage of aseptic cultures (88.57%) while, the treatment T2 recorded lowest (20%) establishment of aseptic culture. Among the various combinations, MS medium supplemented with 6 mg/L BAP + 0.5 mg/L NAA showed highest percentage of culture establishment (60%). The media combination of MS + 6 mg/L BAP + 0.5 mg/L NAA + 0.1 g/L Ascorbic acid recorded the highest percentage (88.89%) of shoot induction. Also, it showed minimum (15.33) days for shoot induction. The same media combination found effective for the highest average number of multiple shoot bud induction (5.33).
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