National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Analysis and comparison of the transcriptome of human hepatocytes infected with the Yellow Fever virus to verify the transcriptional profile of both genotypes, in response to viral infection. The sample used corresponds to strains of YFV code H622205, from the state of Goiás-GO (Brazil), a fatal human case isolated from the patient's blood and serum. The techniques of cell cultures, extraction of viral RNA, sequencing and analysis of transcriptome, as well as the pre-processing of both the RNA and transcriptome libraries, mapping and abundance of transcripts and statistical analyzes were all performed in the Arbovirology Section of the Institute Evandro Chagas. According to the viral titration, there is a greater replication of Genotype II compared to Genotype I. In both infections, we noticed that the moment of the lowest viral load is 24 hpi and the highest viral load is 96 hpi. Regarding the VFA genotype II infecting HepG2, the same tests were carried out, but the significance of the statistical test for differential expression was less than that found in genotype II.A statistical test of formation of “HeatMap” was also performed by the RSEN software. The present study emphasize that this work has great value in clarifying differences in the infection of genotype I and II of YFV in human hepatocytes, in addition to being a milestone, for presenting the first transcriptome of both genotypes of YFV performed in hepatocytes.
Citations |
 |
 |
 |
 |
