National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The importance of microbial chitinase has gained much interest because on the one hand, it reduces environmental hazards and on the other hand it increases production of industrially important value-added products. Hence the present study was conducted with the objective to isolate, screen and characterize chitinase producing fungi from soil samples. In the present study ten fungal isolates have been evaluated for the production of extracellular chitinase and selected one potent isolate based on maximum chitin hydrolysis and identified as Fusarium sp. SS06. Fusarium sp. KS06 has been used for the production of chitinase under submerged fermentation and partially purified and characterized. Fusarium sp. showed maximum enzyme production of at 40°C; pH 8.0 with 1.0 ml of inoculum size. Chitinase enzyme produced from Fusarium sp. was partially purified by 80% ammonium sulphate saturated and molecular weight was determined as ~38 kDa. The partially purified enzyme’s maximum activity at 25°C and pH 9.0. The partially purified chitinase was able to hydrolyse 100% of its natural substrate colloidal chitin. Whereas with regards to starch and casein only chitin hydrolysed only 30% and 21% respectively. In conclusion the extracellular chitinase producing fungi Fusarium sp. was isolated from soil sample and cultural conditions for the production of chitinase under submerged fermentation were optimized. The partially purified alkaline chitinase from Fusarium sp. can be a used as potential biocontrol agent.
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