International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:3, March, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 211-223

Incorporation of BLB Resistance Genes into Basmati Rice Variety CSR-30 (Oryza sativa L.) through Marker Assisted Selection
Nikita Baliyan1,2*, Reema Rani1,3, Kirti Mehta1, Lalit Kumar2 and K. S. Boora1
1CCS Haryana Agricultural University, Hisar, Haryana, India
2Department of Biosciences, College of Applied Education and Health Sciences,Roorkee Road, Meerut, India
3ICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan, India
*Corresponding author

In Asia, rice is the most important, widely consumed staple food for a large part of the world's human population. Bacterial leaf blight (BB), caused by the bacterium, Xanthomonas oryzae pv. oryzae (Xoo) is one of the major disease affecting Basmati rice production in India. CSR-30 is a very popular high yielding, salt tolerant Basmati variety widely grown in Haryana, India but highly susceptible to BB. In the present study, therefore, we successfully introgressed two BB resistance genes (Xa21 and xa13) from BB resistant donor variety Pusa Basmati-1460 into the BB susceptible variety CSR-30 through marker assisted selection (MAS). Hundred and fourteen selected BC1F1 pyramided genotypes were tested for BB resistance at maximum tillering stage against Xoo strain prevalent in Kaul, Haryana. The double resistance genes pyramided genotypes imparted enhanced resistance as expressed by reduced mean lesion length in comparison to genotypes with individual genes. A total of 111 simple sequence repeat (SSR) markers out of 428 representing whole of the genome were found polymorphic between parental genotypes, CSR-30 and Pusa Basmati-1460. Background analysis using 111 polymorphic SSR markers revealed that recurrent parent genome (RPG) recovery ranged up to 83% among twenty five BC1F1 two-gene pyramided genotypes. Based on BB reaction, agronomic evaluation, grain quality and percentage recovery of RPG, four genotypes viz., P-C-70, P-C-123, P-C-142 and P-C-149 were found promising. These promising genotypes possessing yield traits similar or at par with the recurrent Basmati parent CSR-30 were identified and advanced to BC2F1 generation. The experimental results of this work demonstrate the effectiveness of MAS for successful introgression of the two BB resistance genes from Pusa Basmati-1460 into CSR-30.

Keywords: Rice, Bacterial leaf blight, Gene pyramiding, Marker assisted selection, Xanthomonas oryzae pv. oryzae

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How to cite this article:

Nikita Baliyan, Reema Rani, Kirti Mehta, Lalit Kumar and Boora, K. S. 2021. Incorporation of BLB Resistance Genes into Basmati Rice Variety CSR-30 (Oryza sativa L.) through Marker Assisted Selection.Int.J.Curr.Microbiol.App.Sci. 10(3): 211-223. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.