National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In Asia, rice is the most important, widely consumed staple food for a large part of the world's human population. Bacterial leaf blight (BB), caused by the bacterium, Xanthomonas oryzae pv. oryzae (Xoo) is one of the major disease affecting Basmati rice production in India. CSR-30 is a very popular high yielding, salt tolerant Basmati variety widely grown in Haryana, India but highly susceptible to BB. In the present study, therefore, we successfully introgressed two BB resistance genes (Xa21 and xa13) from BB resistant donor variety Pusa Basmati-1460 into the BB susceptible variety CSR-30 through marker assisted selection (MAS). Hundred and fourteen selected BC1F1 pyramided genotypes were tested for BB resistance at maximum tillering stage against Xoo strain prevalent in Kaul, Haryana. The double resistance genes pyramided genotypes imparted enhanced resistance as expressed by reduced mean lesion length in comparison to genotypes with individual genes. A total of 111 simple sequence repeat (SSR) markers out of 428 representing whole of the genome were found polymorphic between parental genotypes, CSR-30 and Pusa Basmati-1460. Background analysis using 111 polymorphic SSR markers revealed that recurrent parent genome (RPG) recovery ranged up to 83% among twenty five BC1F1 two-gene pyramided genotypes. Based on BB reaction, agronomic evaluation, grain quality and percentage recovery of RPG, four genotypes viz., P-C-70, P-C-123, P-C-142 and P-C-149 were found promising. These promising genotypes possessing yield traits similar or at par with the recurrent Basmati parent CSR-30 were identified and advanced to BC2F1 generation. The experimental results of this work demonstrate the effectiveness of MAS for successful introgression of the two BB resistance genes from Pusa Basmati-1460 into CSR-30.
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