International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:3, March, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 118-128

Isolation and Partial Characterization of Bacteriophage against Salmonella gallinarum
Shumaila Malik, Renu Chauhan, Lahari Laddika, Salauddin Qureshi*,Bablu Kumar and A. K. Tiwari
Division of Biological Standardization, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, India
*Corresponding author

A lytic bacteriophage (SGP-1) against Salmonella gallinarum (S. gallinarum) was isolated by an enrichment protocol from the sewage water of a poultry farm. The presence of bacteriophage was observed by spot test over the bacterial lawn and double agar overlay assay. Isolated phage was able to produce clear, circular plaques of 2-3mm diameter on S. gallinarum lawn culture. This Bacteriophage also had a lytic effect on Salmonella typhimurium. The bacteriophage SGP-1 was found to be stable at a temperature range between 4oC to 60oC for 30 min and able to survive in a wide range of pH between 2 to 11. The characterization of the bacteriophage would help establish a basis for adopting the application of the most effective bacteriophage therapeutics to control bacterial infection of poultry. 

Keywords: Salmonella Gallinarum, Bacteriophage, Double agar overlay, Host range

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How to cite this article:

Shumaila Malik, Renu Chauhan, Lahari Laddika, Salauddin Qureshi, Bablu Kumar and Tiwari, A. K. 2021. Isolation and Partial Characterization of Bacteriophage against Salmonella gallinarum.Int.J.Curr.Microbiol.App.Sci. 10(3): 118-128. doi:
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