National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A reliable protocol for efficient shoot induction in brinjal cv. CO 2 was developed. The effects of different plant growth regulators and explant types in in-vitro shoot induction were studied. The 12-15 days old cotyledonary leaves showed the maximum shoot induction. The regeneration efficiency was significantly increased by using Thidiazuron (TDZ) in culture medium as a growth regulator. Following the standardized regeneration protocol, Agrobacterium-mediated transformation of brinjal cv. CO 2 was optimized. Cotyledonary leaf bits were used for co-cultivation with the Agrobacterium strain LBA4404 carrying pCAMBIA 2301 vector. A transformation efficiency of 33.5% was obtained by using the cotyledonary leaves as explants. The transformation efficiency was significantly enhanced when the explants were cocultivated on medium overlaid with Whatman No.1 filter paper.
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