National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Blood stream infections are very common in the paediatric age group and these are one of the common causes of morbidity and mortality in neonates and children. Objectives of the study are to isolate the different organisms from Blood cultures of Neonates by BACTEC method and know their Antibiotic susceptibility patterns. We processed 100 Blood samples of the Neonates from PICU of NIMS Medical College and Hospital between January and June 2016 (06 months). Samples were collected in BACTEC PEDS PLUS vials with all aseptic precautions. The vials were placed in the BACTEC 9050 and cultured. The positive vials were further plated on BA, MacConkey Agar looked for the growth and identified by necessary biochemical reactions and antibiotic susceptibility is done by Kirby-Bauer method. Out of 100 blood culture samples, 58 were positive. Out of 58, 18 yielded CoNS (31.03%), 12- Klebsiella spp (20.68%), 04 - Staphylococcus aureus (6.89%), 06 - Enterococcus spp (10.34%), 12- Enterobacter spp (20.68%), and 02 each of E. coli (3.44%), Citrobacter (3.44%) and Acinetobacter (3.44%). Significantly high rates of Gram Negative bacterial infections were seen followed by Gram Positives. Automated Blood Culture systems significantly reduced the time required for processing of samples and also facilitated the yield of diverse organisms. Antibiotic Susceptibility patterns also showed Gram Negative bacteria to be less sensitive than Gram Positive bacteria.
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