International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1533-1540

Isolation and Characterization of Plant Growth Promoting Endophytic Diazotrophic Bacteria from Wheat Genotypes and their Influence on Plant Growth Promotion
Devendra Singh1, Neelam Geat3, Mahendra Vikram Singh Rajawat2, Radha Prasanna1, Anil Kumar Saxena2 and Rajeev Kaushik1*
1Division of Microbiology, ICAR-Indian Agricultural Research Institute,New Delhi – 110012, India
2ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur,
Mau Nath Bhanjan, Uttar Pradesh – 275103, India
3Division of Plant pathology, ICAR-Indian Agricultural Research Institute,
New Delhi – 110012, India
*Corresponding author

Nitrogen (N) is an essential element for the growth and yield of wheat. Some endophytic bacteria can fix N2 from the air and convert to nitrogen compounds that can be utilized by plants. In this study, endophytic bacteria were isolated from one-month-old seedlings of 13 wheat genotypes growing without nitrogen fertilizer in the ICAR –IARI field. A total of 23 diazotrophic endophytes were isolated from 13 wheat genotypes used. Nitrogenase enzyme activities of endophytes were measured by acetylene reduction assay (ARA). Among 23 isolates, 17 isolates showed the IAA production and three isolates exhibited siderophore production ability. 13 isolated diazotrophic endophytes give clear halo zone around the colony on tricalcium phosphate containing Pikovaskaya’s agar plates. None of them exhibited HCN and ammonia production ability. Nitrogenase activity of isolates was ranged from 5 to 30 nmol C2H4/mg protein/h. Endophytes inoculation significantly increase the root length, root fresh weight and root dry weight. Likewise, endophytes inoculation also showed significant results on fresh weight and dry weight of shoot. Both the endophytes were identified as Azospirillium sp. by 16S rRNA gene sequencing.

Keywords: Endophytic bacteria, Nitrogenase enzyme, HCN, Phosphate solubilization, Azospirillium sp
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How to cite this article:

Devendra Singh, Neelam Geat, Mahendra Vikram Singh Rajawat, Radha Prasanna, Anil Kumar Saxena and Rajeev Kaushik. 2017. Isolation and characterization of Plant growth promoting endophytic diazotrophic bacteria from wheat genotypes and their influence on plant growth promotionInt.J.Curr.Microbiol.App.Sci. 6(4): 1533-1540. doi: