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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 3026-3030
DOI: https://doi.org/10.20546/ijcmas.2020.912.358


Isolation, Molecular Identification and Antibiogram of Streptococcus dysgalactiae Isolates Recovered from Pigs
Renu Chauhan1*, Lahari Laddika1, M. Dinesh2, Bhutediya Jitendrakumar Maganbhai1, Shumaila Malik1, M. Sahoo2, Salauddin Qureshi1 and A. K. Tiwari1
1Division of Biological Standardisation, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, India
2Division of Pathology, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, India
*Corresponding author
Abstract:

Streptococcus dysgalactiae is a pyogenic species pathogenic both for animals and humans. It is an emerging pathogen classified as β haemolytic streptococci belonging to Lancefield group C and G. Present study was undertaken to isolate, identify and analyse the antibiogram of S. dysgalactiae isolates recovered from pigs. A total of 53 samples were collected from slaughtered pigs, out of which 35 isolates confirmed as Gram positive streptococci microscopically. Biochemically 31 isolates were identified as Streptococcus dysgalactiae subspequisimilis and 4 as group L Streptococci by using API STREP 20multitest kit identification system. PCR targeting 23S rDNA gene sequence which confirmed 31 isolates as Streptococcus dysgalactiae. Antibiogram of these isolates was done using 13 antibiotic discs for testing antimicrobial sensitivity using standard disc diffusion method. All the isolates were highly sensitive to β lactams followed by fluoroquinolones, tetracyclines, least susceptible to sulphonamides, aminoglycosides and were complete resistant to neomycin. Hence the study highlights the presence of emerging opportunistic pathogen S. dysgalactiae in pigs and its decreasing susceptibility towards aminoglycosides. This study is significant as microorganism Streptococcus dysgalactiae subsp equisimilis is an emerging pathogen in humans as well.


Keywords: Molecular identification Antibiogram, Streptococcus dysgalactiae, Pigs
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How to cite this article:

Renu Chauhan, Lahari Laddika, M. Dinesh, Bhutediya Jitendrakumar Maganbhai, Shumaila Malik, M. Sahoo, Salauddin Qureshi and Tiwari, A. K. 2020. Isolation, Molecular Identification and Antibiogram of Streptococcus dysgalactiae Isolates Recovered from PigsInt.J.Curr.Microbiol.App.Sci. 9(12): 3026-3030. doi: https://doi.org/10.20546/ijcmas.2020.912.358