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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 2378-2390
DOI: https://doi.org/10.20546/ijcmas.2020.912.283


Optimization of Medium Composition and Cultural Conditions for Enhanced Endoxylanase Production from Aspergillus fumigatus SKF-4 using Response Surface Methodology
Dolmani Amat1,2* and Livleen Shukla1
1ICAR- Indian Agricultural Research Institute, Division of Microbiology, New Delhi, India
2ICAR-Indian Institute of Soil Science, Nabibagh, Berasia Road, Bhopal, India
*Corresponding author
Abstract:

Xylanases have multiple uses in industries for obtaining value added products from lignocellulosic biomass. In this study, wheat bran concentration, pH of the medium and incubation temperature were optimized using Rotatable central composite design based Response surface methodology for enhancing the production of endoxylanase from Aspergillus fumigatus SKF-4. In the model that was developed, linear and quadratic effect of all the independent variables and interaction effect of wheat bran concentration and the medium pH were significant. Model predicted optimized conditions as wheat bran- 2.5 (%w/v), pH- 5.7 and temperature- 32°C with endoxylanase production as 60.3 IU/ml. Endoxylanase production under experimental condition was 60.8 IU/ml which was at par with the predicted value. Under optimized conditions endoxylanase production was 1.5 times more than the unoptimized one. Response surface methodology could help in optimization of process parameters for enhanced production of endoxylanase from Aspergillus fumigatus SKF-4.


Keywords: Wheat bran, Xylanase, Independent variables, Quadratic equation
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How to cite this article:

Dolmani Amat and Livleen Shukla. 2020. Optimization of Medium Composition and Cultural Conditions for Enhanced Endoxylanase Production from Aspergillus fumigatus SKF-4 using Response Surface Methodology.Int.J.Curr.Microbiol.App.Sci. 9(12): 2378-2390. doi: https://doi.org/10.20546/ijcmas.2020.912.283