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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 2072-2084
DOI: https://doi.org/10.20546/ijcmas.2020.912.244


Selection of Monokaryotic Isolates of Pleurotus sajor-caju, Pleurotus flabellatus and Pleurotus djamor for their Dikaryotization
Aparajita Dhar1, K. Sinijadas2, Pulak Bhaumik1,Suman Datta3 and Sekhar Bandyopadhyay1*
1Department of Plant Pathology, Uttar Banga Krishi Viswavidyalaya, Pundibari, Coochbehar – 736165, West Bengal, India
2Department of Plant Pathology, College of Agriculture, Kerala Agricultural University, Vellayani, Thiruvananthapuram, Kerala – 695522, India
3Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur,
Nadia – 741252, West Bengal, India
*Corresponding author
Abstract:

The experiment was conducted at UBKV, Pundibari, Coochbehar during the academic year of 2016 – 2018 to find out the monokaryotic isolates of P. flabellatus, P. sajor-caju and P. djamor (oyster pink or OP) for its dikaryotization with the help of rapidity in the growth performance. Eighteen, six and five monokaryotic isolates of P. flabellatus (PFM), P. sajor-caju (PSCM) and P. djamor (OPM), respectively were grown on Malt Extract Agar media (MEA) for testing their rapidity in radial growth. After the selection of monokaryotic isolates growth performance was again tested for selection of dikaryotic culture on Malt Extract Agar (MEA) media. In MEA media, rapid radial growth of monokaryotic isolates viz., PF8M, PF11M, PF16M and PF17M for P. flabellatus, PSC3M and PSC5M for P. sajor-caju, OP1M and OP5M for P. djamor (oyster pink or OP) was finally selected for dikaryotization (hybridization). Highest growth rate on MEA was observed in interspecific hybrids of PF × PSC12 (18.50 mm/day), PF × PSC16 (18 mm/day), PF × PSC17 (17.50 mm/day), PF × PSC8 (17 mm/day) and PF × PSC2 (16.50 mm/day), respectively. Interspecific hybrids of OP × PSC5 (21.50 mm/day) followed by OP × PSC4 (21 mm/day), OP × PSC8 (20.50 mm/day) and OP × PSC12 (19.50 mm/day) and hybrids of OP × PF 5, OP × PF1, OP × PF2, OP × PF4 shows the growth rate above 18 mm/day. Dikaryotic mycelium showed highest growth rate as compared to monokaryotic isolates of P. flabellatus, P. sajor-caju and P. djamor (oyster pink).


Keywords: Pleurotus flabellatus, Pleurotus sajor-caju, Pleurotus djamor, Monokaryotic, Dikaryotization, Malt extract agar
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How to cite this article:

Aparajita Dhar, K. Sinijadas, Pulak Bhaumik, Suman Datta and Sekhar Bandyopadhyay. 2020. Selection of Monokaryotic Isolates of Pleurotus sajor-caju, Pleurotus flabellatus and Pleurotus djamor for their Dikaryotization.Int.J.Curr.Microbiol.App.Sci. 9(12): 2072-2084. doi: https://doi.org/10.20546/ijcmas.2020.912.244