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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 995-999
DOI: https://doi.org/10.20546/ijcmas.2020.912.120


Evaluation of Suitable Culture Media for Growth of Rhizoctonia bataticola Causing Dry Root Rot of Chickpea (Cicer arietinum)
Anurag Shukla1*, Ved Ratan1, R K Mishra2, U.K. Tripathi1, Krishna Kumar2, D. R. Singh3, R.K. Pathak4, Javed Bahar1 and Anjani Kumar Singh5
1Department of Plant Pathology,
3Department of Entomology,
4Department of Soil Science & Agri. Chemistry,
5Department of Agri. Economics & Statistics, C.S.A.U.A &T, Kanpur-2, Uttar Pradesh, India
2Division of Crop Protection, IIPR, Kanpur-2, Uttar Pradesh, India
*Corresponding author
Abstract:

Rhizoctonia bataticola (Taub.) Butler is the causal organism of dry root rot of chickpea. Its Pycnidial stage is found in Macrophomina phaseolina (Tassi) Goid. It is soil inhabiting fungal pathogen. Keeping in view the consequentiality of the disease due to transmute in climatic conditions, studies were conducted on distribution of the disease, cultural, morphological, pathological and molecular diversity. This research was conducted at Department of Plant Pathology, CSAUA & T, Kanpur, in collaboration with Indian Institute of Pulses Research, Kanpur. In this research, dry root rot affected samples were collected from different farms of Uttar Pradesh. A total of fifty R. bataticola isolates of were isolated from these samples. Eight different culture media were used in this study for evaluation of the best for growth of Rhizoctonia bataticola. The fungus R. bataticola gives maximum cultural growth on potato dextrose agar (88.63 mm) followed by corn meal agar 78.36 mm and oat meal agar 73.36 mm while potato dextrose rose bengal agar showed least growth of 28.50 mm in solid media. Among the eight liquid broth media tested, potato dextrose broth revealed the highest mean mycelial dry weight (158.50 mg) followed by corn meal broth (130.90 mg), oat meal broth (127.60 mg), V-8 juice broth (119.10 mg) while potato dextrose rose bengal broth gives the least mycelial dry weight (53.60 mg). Production of sclerotia was maximum in PDA and least in SDA.


Keywords: Rhizoctonia bataticola, Cultural growth, Dry weight, Broth, Macrophomina phaseolina
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How to cite this article:

Anurag Shukla, Ved Ratan, R K Mishra, U.K. Tripathi, Krishna Kumar, D. R. Singh, R. K. Pathak, Javed Bahar and Anjani Kumar Singh. 2020. Evaluation of Suitable Culture Media for Growth of Rhizoctonia bataticola Causing Dry Root Rot of Chickpea (Cicer arietinum).Int.J.Curr.Microbiol.App.Sci. 9(12): 995-999. doi: https://doi.org/10.20546/ijcmas.2020.912.120