National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Puruvate formate lyase (PFL) of Staphylococcus aureus is an important protein which gets expressed mainly as an adaptive response for their survival in the host mammary tissue by way of producing biofilm. This adaptive mechanism of S. aureus mainly contributes to sub-clinical, chronic bovine mastitis which often becomes challenging to treat with conventional antibiotics and thereby the prevention of further spread of infection in the herd becomes difficult. The present preliminary study is an attempt to clone and characterize 609 bp antigenic site of pyruvate formate lyase gene of S. aureus associated with bovine mastitis. The partial PFL gene cloned using TA cloning vector showed 98-99% identity with the reference sequence available in the Genbank (MK695632). The cloned insert with overhangs released by restriction enzyme digestion can be used to obtain the antigenic protein by using pET 32a+ expression vector system. The antibodies to pyruvate formate lyase were obtained in the serum of cows associated with S. aureus bovine mastitis which was as an important finding in Serological Proteome Analysis (SERPA). Therefore the protein expressed from the cloned partial PFL gene can be used for the development of immunoassay kit for early diagnosis and also as a vaccine candidate for the control and prevention of S. aureus associated bovine mastitis.
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