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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 256-265
DOI: https://doi.org/10.20546/ijcmas.2020.912.034


Expression Analysis of DNA Methyltransferases among Children Infected with Mycobacterium tuberculosis
Gayathri Pandurangan1, Noyal Mariya Joseph2, Mahadevan Subramaniyan1*
1Department of Paediatrics, Jawaharlal Institute of Postgraduate Medical Education and
Research (JIPMER), Puducherry, India
2Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India
*Corresponding author
Abstract:

DNA Methylation plays a significant role in the prompt functioning of the immune system during the pathogenesis of Mycobacterium tuberculosis among paediatric population. Methylation is catalysed and regulated by DNA Methyltransferases (DNMTs). DNMT1, DNMT3A and DNMT3B are the essential DNMTs involved in the active transfer of Methyl group to the DNA. Thus, the upregulated DNMTs could pave way for hypermethylation of salient immune molecules and thereby transcriptionally inactivating the immune molecules involved in eliciting primary immune response. This study was aimed to determine the expression levels of DNA Methyltransferases namely DNMT1, DNMT3A and DNMT3B among children infected with M.tb and compared with healthy children as controls. A total of 60 cases and 60 controls were recruited to estimate and compare the expression levels of DNMTs. The expression of the mRNAs coding DNMT1, DNMT3A and DNMT3B were analysed by Real-time Quantitative PCR assay. Comparatively among children infected with M.tb, the expression of DNMT1, DNMT3A and DNMT3B were upregulated [DNMT1 - 0.72(IQR 0.27-1.0); DNMT3A- 0.52(IQR 0.42-.76)& DNMT3B-0.85(IQR 0.74-0.97)] while healthy controls showed downregulated pattern of expression[DNMT1-0.24 (IQR 0.20-.49); DNMT3A- 0.22 (IQR 0.14-.43)& DNMT3B- 0.37(IQR0.30-0.74)]. Thus, the upregulated pattern of DNMTs expression among cases would have assisted pathogenesis by deregulating the transcriptional machinery of host immune system through DNA methylation based Epigenetic modulation.


Keywords: DNA Methylation, DNA Methyltransferases, DNMT in TB, Paediatric TB& Epigenetics in TB
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How to cite this article:

Gayathri Pandurangan, Noyal Mariya Joseph, Mahadevan Subramaniyan. 2020. Expression Analysis of DNA Methyltransferases among Children Infected with Mycobacterium tuberculosis.Int.J.Curr.Microbiol.App.Sci. 9(12): 256-265. doi: https://doi.org/10.20546/ijcmas.2020.912.034