International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:12, December, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(12): 235-239

Mechanical Transmission of Leaf Crinkle Disease in Urdbean (Vigna mungo (L.) Hepper) by Sprout Seed Abrasion Method
T. Ramya Teja1*, T. K. S. Latha1, T. Priyanga1, K. Prabakar1, G. Karthikeyan1, M. Raveendran2 and V. Balasubramani2
1Department of Plant Pathology,
2Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India
*Corresponding author

Urdbean or blackgram (Vigna mungo (L.) Hepper) is one of the important leguminous cropscultivated in India, Pakistan and South-East Asian countries. Among the biotic stresses, Urdbean leaf crinkle disease (ULCD), is economically significant and devastating in major urdbean growing countries. ULCD causes severe crinkling and rugosity of lamina, stunting of plants, malformation of buds inflicting heavy yield losses. Due to occurrence of ULCD as mixed infections along with other major virus diseases of pulses, maintenance of pure inoculum under glasshouse conditions is a prerequisite. In the present study, seeds were mechanically sap inoculated by sprout seed abrasion method. Influence of artificial inoculation on ULCD incidence has been studied in CO5 cultivar of blackgram. Symptoms started appearing 30-35 DPI and disease incidence was up to43.3%. This protocol will help in maintenance of pure inoculum of ULCD under glasshouse conditions and further investigations are under process to standardize efficient protocol to obtain higher transmission efficiency.

Keywords: Mechanical Inoculation, Sprout seed, Urdbean, Leaf crinkle, ULCD

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How to cite this article:

Ramya Teja, T., T. K. S. Latha, T. Priyanga, K. Prabakar, G. Karthikeyan, M. Raveendran and Balasubramani, V. 2020. Mechanical Transmission of Leaf Crinkle Disease in Urdbean (Vigna mungo (L.) Hepper) by Sprout Seed Abrasion Method.Int.J.Curr.Microbiol.App.Sci. 9(12): 235-239. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.