National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Urdbean or blackgram (Vigna mungo (L.) Hepper) is one of the important leguminous cropscultivated in India, Pakistan and South-East Asian countries. Among the biotic stresses, Urdbean leaf crinkle disease (ULCD), is economically significant and devastating in major urdbean growing countries. ULCD causes severe crinkling and rugosity of lamina, stunting of plants, malformation of buds inflicting heavy yield losses. Due to occurrence of ULCD as mixed infections along with other major virus diseases of pulses, maintenance of pure inoculum under glasshouse conditions is a prerequisite. In the present study, seeds were mechanically sap inoculated by sprout seed abrasion method. Influence of artificial inoculation on ULCD incidence has been studied in CO5 cultivar of blackgram. Symptoms started appearing 30-35 DPI and disease incidence was up to43.3%. This protocol will help in maintenance of pure inoculum of ULCD under glasshouse conditions and further investigations are under process to standardize efficient protocol to obtain higher transmission efficiency.
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