'' '' '' '' '' Valorization of the Mucilage Juice of Cocoa Beans for the Production of a Biopesticide based on <em>Bacillus thuringiensis var.kurstaki</em> HD-1
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 3600-3610
DOI: https://doi.org/10.20546/ijcmas.2020.911.431


Valorization of the Mucilage Juice of Cocoa Beans for the Production of a Biopesticide based on Bacillus thuringiensis var.kurstaki HD-1
ACHI Stanislas Maurice Akpo1,4*, YAPO Atse Alain1,4, DJENI N' dede Théodore2, GADJI Alahou André Gabaze1,6, COULIBALY Kalpy Julien4, GNEPE Jean Robert5, YAPO Ossey Bernard1,3 and TYAGI Dayal Rajeshwar5
1Laboratory of Environmental Sciences (LSE), Training and Research Unit in Environmental Sciences and Management (UFR-SGE), Nangui Abrogoua University 02 BP 801 Abidjan 02, Côte d’Ivoire
2Biotechnology and Food Microbiology Laboratory, Department of Food Science and Technology, University of Nangui Abrogoua, Abidjan, 02 BP 801, Côte d’Ivoire
3Central Laboratory for the Environment of the Ivorian Antipollution Center (LCE-CIAPOL) 20 BP 650 Abidjan 20, Côte d'Ivoire
4Environment and Health Laboratory, Environmental Microbiological and
Control Unit of the Pasteur Institute of Côte d'Ivoire 01 BP 490 Abidjan 01
5Laboratory for Bioconversion of Wastewater and Sewage Sludge into High Added Value Products, National Institute for Scientific Research (INRS-Water Earth and Environment), University of Quebec, Quebec, Canada
6Technology Laboratory of the National Agronomic Research Center (CNRA), 01 BP 1740 Abidjan 01Côte d'Ivoire
*Corresponding author
Abstract:

The use of microbial pesticides in agriculture has someadvantages, but their production ina non-conventional experimental tests constituate a challenge. This study purpose is to evaluate  the potential of cocoa mucilage juice. The mucilage juice of cocoais a substratum, that has been used as an alternative growing test and associated to bacteria such as Bacillus thuringiensis var. kurstaki HD-1 (Btk HD-1) for the production of a biopesticide. After determining the physico-chemical characteristics of the mucilage juice, different combinations has been made with 2% (M2%), 4% (M4%), 6% (M6%) and 7% (M7%) cane molasses were established, then inoculated with 2% of the inocula and incubated at 30 °C for 48 hours under agitation. The results showed that the substratum contained nitrogen (1.68g/L) and carbon (9.9 g/L), which  has an important mineral such as potassium (87.3 mg/L), phosphorus (38 mg/L) and magnesium (25.42 mg/L). The growth of Btk HD-1 in cocoa mucilage juice with 2% molasses (M2%) was the highest (p < 0.0001) with average concentration of 1.6×109 CFU/mL for cells and 2.3×109 CFU/mL for spores. This combination therefore offers good prospects for the production of the Btk HD-1 biopesticide.


Keywords: Mucilage juice, Cane molasses, biopesticide, Bacillus thuringiensis var. kurstaki HD-1, liquid fermentation
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How to cite this article:

ACHI Stanislas Maurice Akpo, YAPO Atse Alain, DJENI N' dede Théodore, GADJI Alahou André Gabaze, COULIBALY Kalpy Julien, GNEPE Jean Robert, YAPO Ossey Bernard and TYAGI Dayal Rajeshwar. 2020. Valorization of the Mucilage Juice of Cocoa Beans for the Production of a Biopesticide based on Bacillus thuringiensis var.kurstaki HD-1.

Int.J.Curr.Microbiol.App.Sci. 9(11): 3600-3610. doi: https://doi.org/10.20546/ijcmas.2020.911.431
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