International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 3590-3599

Use of Sugar Cane Molasses Enriched with Yeast Extract for the Production of Biopesticide from Bacillus thuringiensis var. kurstaki HD-1
YAPO Atsé Alain1,4*, ACHI Stanislas Maurice Akpo1,4, DJENI N'dédé Théodore2, GADJI Alahou André Gabaze6, COULIBALY Kalpy Julien4, GNEPE Jean Robert5, YAPO Ossey Bernard1,3 and TYAGI Dayal Rajeshwar5
1Environment Sciences Laboratory, Training and Research Unit in Environmental Sciences and Management, 2Biotechnology and Food MicrobiologyLaboratory, Department of Food Science and Technology, University of NanguiAbrogoua, 02 BP 801, Côte d’Ivoire
3CentralLaboratory of the Environment of the Ivorian Anti-Pollution Center, BP 650 Abidjan 20, Côte d’Ivoire
4Environment and healthLaboratory, Control Unit and Environmental Microbiology, Pasteur Institute of Côte d’Ivoire
5Laboratory of Bioconversion of Wastewater and SewageSludgeinto High Added Value Products, National Institute of Scientific Research, University of Quebec, Canada
6TechnologyLaboratory of the National Center for Agricultural Research01 BP 1740 Abidjan 01, Côte d’Ivoire
*Corresponding author

Bacillus thuringiensis subspecies kurstaki HD-1 (Btk HD-1) has been widely used for five decades as a leading biopesticide for its ecological action. However, this biopesticide often has challenges related toits costs of production and its formulation. The purpose of this work was to evaluate a novel low-cost Btk HD-1-based biopesticide production method using sugar cane molasse enriched with yeast extracts. After determining their content, Four growth media containing variable proportion of molasses and yeast extracts were formulated, then inoculated with (BtkHD-1) and incubated to 30 ± 1 °C. The results showed that the cane molasses were enriched in minerals such as Mn (142.08 mg/kg), Cu (144.1 mg/kg), Fe (108.18 mg/kg), Ca (97.2 mg/kg), K (79.7 mg/kg), Mg (73.96 mg/kg), Na (51.24 mg/kg) and Zn(17.7 mg/kg). Moreover, the quantity of yeast extracts had an impact on the growth rate and sporulation of Bt. Overall, the media with 200 g of yeast extract was the most suitable growth medium for Btk HD-1.Sugar cane molasses-based culture medium enriched with yeast extract could replace soy flour for its availability and low-cost.

Keywords: Bacillus thuringiensis, Biopesticide, Fermentation, Molasses, Yeast extracts

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How to cite this article:

Yapo Atsé Alain, Achi Stanislas Maurice Akpo, Djeni N'dédé Théodore, Gadji Alahou André Gabaze, Coulibaly KalpyJ ulien, Gnepe Jean Robert, Yapo Ossey Bernard and Tyagi Dayal Rajeshwar. 2020. Use of Sugar Cane Molasses Enriched with Yeast Extract for the Production of Biopesticide from Bacillus thuringiensis var. kurstaki HD-1.Int.J.Curr.Microbiol.App.Sci. 9(11): 3590-3599. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.