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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1184-1192
DOI: https://doi.org/10.20546/ijcmas.2017.604.145


Characterization and Identification of Phytate Solubilizing Yeasts Isolated from Food Grains
P.D. Savita*, V.C. Suvarna, T. Annu, A.N. Balakrishna, B. Kanchanashri and M. Yallappa
Department of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bangalore, Karnataka, India
*Corresponding author
Abstract:

Phytase activity of bacteria, yeasts and moulds isolated from different ecosystems have been identified and studied by several researchers. The present study focused on isolation and identification of phytate solubilizing yeasts associated with freshly soaked cereals, pulses, condiments and oilseeds. Isolation on MRS-Calcium phytate at pH 6.2 ±0.2 resulted in a high diversity of microorganisms developing clear zones of phytate hydrolysis. Microscopic study revealed identification of three groups of phytate solubilizing organisms (viz., yeasts, bacteria and moulds); only yeasts were selected for the following study. Screening on phytase screening medium (phytate as the only source of P) confirmed the phytase activity of a few yeasts species within 2 to 4 h of inoculation. Morphological, physiological and 18SrRNA analysis confirmed that yeasts isolates belonged to the genera; Meyerozyma, Saccharomyces, Geotrichum and Rhodotorula species. This preliminary investigation has discovered a novel enzyme activity in Meyerozyma, Geotrichum and Rhodotorula which has not been reported earlier. Detail analysis of these identified strains with respect to phytate degradation in vitro and in vivo is being studied in our laboratory to check the potential application of its enzyme ‘phytase’ in the feed technology.


Keywords: Phytate solubilizing yeasts; Food grains; Characterization; Identification.
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How to cite this article:

Savita, P.D., V.C. Suvarna, T. Annu, A.N. Balakrishna, B. Kanchanashri and Yallappa, M. 2017. Isolation, Characterization and Identification of Phytate Solubilizing Yeasts Dwelling Food GrainsInt.J.Curr.Microbiol.App.Sci. 6(4): 1184-1192. doi: https://doi.org/10.20546/ijcmas.2017.604.145