National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The objectives of this study were to identify the prevalence and the sources of contamination of Listeria monocytogenes in BTM of examined 5 farms and to assess the used of the chromogenic media and API technique as rapid confirmation for the presence of this pathogen. The present study was carried on BTM and farm environmental samples collected from 5 dairy farms in Egypt. The samples were examined for the incidence of Listeria species using conventional isolation method and the identification of L. monocytogenes by the using of chromogenic media, API test and PCR technique. The detection method based on PCR amplification of the hlyA gene revealed that the incidence of Listeria monocytogenes were 6.66%, 14%, 10%, 8%, 5.6%, 0% and 0% in BTM, feces, bedding, water troughs, teat skin, milking equipment and hand swabs, respectively. L. monocytogenes was isolated from 3 out of 5 farms investigated. Antimicrobial susceptibility was done for all identified strains isolated from BTM against 17 antimicrobial agents. All of the isolates were sensitive to Imipenem(IPM), Penicillin G(P), Ampicillin(AMP), Amoxycillin/clavulanic acid(AMC), Ampicillin/ sulbactam (SAM), Chloramphenicol(C), Levofloxacin(LEV), Cephradine, Ciprofloxacin (CIP), Cefquinome(CEQ), Ofloxacin(OFX) and Amikacin(AK).
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