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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 144-158
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.020


Application of Specific Chromogenic Media and Api Technique for Rapid Confirmation of Listeria monocytogenes in Bulk Tank Milk
and Dairy Farms Environment
S.M. Dabash2*, A.M. Saudi1, H. El Essawy1 and R.H. Hamouda2
1Food Hygiene & Control Department, Faculty of Veterinary Medicine,
Cairo University, Giza, Egypt
2Researcher Mastitis & Neonatal Diseases Res. Department Animal Reproduction
Research Institute (Al-Haram, Giza, Egypt)
*Corresponding author
Abstract:

The objectives of this study were to identify the prevalence and the sources of contamination of Listeria monocytogenes in BTM of examined 5 farms and to assess the used of the chromogenic media and API technique as rapid confirmation for the presence of this pathogen. The present study was carried on BTM and farm environmental samples collected from 5 dairy farms in Egypt. The samples were examined for the incidence of Listeria species using conventional isolation method and the identification of L. monocytogenes by the using of chromogenic media, API test and PCR technique. The detection method based on PCR amplification of the hlyA gene revealed that the incidence of Listeria monocytogenes were 6.66%, 14%, 10%, 8%, 5.6%, 0% and 0% in BTM, feces, bedding, water troughs, teat skin, milking equipment and hand swabs, respectively. L. monocytogenes was isolated from 3 out of 5 farms investigated. Antimicrobial susceptibility was done for all identified strains isolated from BTM against 17 antimicrobial agents. All of the isolates were sensitive to Imipenem(IPM), Penicillin G(P), Ampicillin(AMP), Amoxycillin/clavulanic acid(AMC), Ampicillin/ sulbactam (SAM), Chloramphenicol(C), Levofloxacin(LEV), Cephradine, Ciprofloxacin (CIP), Cefquinome(CEQ), Ofloxacin(OFX) and Amikacin(AK).


Keywords: Bulk tank milk, Listeria monocytogenes, Chromogenic media - API Technique
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How to cite this article:

Dabash, S.M., Saudi, A.M., El Essawy, H. and Hamouda, R.H. 2016. Application of Specific Chromogenic Media and Api Technique for Rapid Confirmation of Listeria monocytogenes in Bulk Tank Milk and Dairy Farms Environment.Int.J.Curr.Microbiol.App.Sci. 5(3): 144-158. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.020