International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 2443-2451

Bacteriological and Molecular Detection with Antimicrobial Resistance Pattern of Major Streptococcus spp. Isolated from Bovine Mastitis
B. B. Javia1*, B. S. Mathapati1, D. B. Barad1, S. N. Ghodasara1, H. H. Savsani2, A.R. Bhadaniya3, D. T. Fefar3, U. D. Patel4 and S. H. Sindhi5
1Department of Veterinary Microbiology,
2Department of Animal Nutrition,
3Department of Veterinary Pathology,
4Department of Veterinary Pharmacology and toxicology,
5Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and A. H., Junagadh Agricultural University, Junagadh, Gujarat, India
*Corresponding author

Mastitis in bovine cause various clinical consequence with much losses in milk quality. Inflammatory infection caused by various bacteria damage the mammary gland and sometimes leads irreversible damage to the udder tissue. Looking to above problem the current study was performed to know the prevalence of major Streptococcus spp. with its timely detection by molecular methods and its antimicrobial resistance patterns. Total 390 bovine milk samples (180 clinically mastitic and 210 from apparently healthy) were studied. Initially samples were screened for major Streptococcus spp. by conventional bacteriological methods of identification. The molecular detection of Streptococcus genus and its major species Viz; Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis was carried out by polymerase chain reaction by targeting Tu (tuf) gene, sip gene, 16 S rRNA gene and pau gene respectively. Based on these overall 16.67 per cent of prevalence was observed for Streptococci. Among the major species, prevalence of S. agalactiae, S. dysgalactiae and S. uberis were found 13.59, 2.31 and 0.77 per cent respectively. These isolates showed highest antimicrobial resistance against amoxicillin- sulbactam (72.25%) followed by ceftriaxone (66.19%) and oxytetracycline (53.12%) while the gentamicin (8.38%) were found least resistant.

Keywords: Bovine Mastitis, Major Streptococcus spp., Molecular Detection, Antimicrobial Resistance

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How to cite this article:

Javia, B. B., B. S. Mathapati, D. B. Barad, S. N. Ghodasara, H. H. Savsani, A. R. Bhadaniya, D. T. Fefar, U. D. Patel and Sindhi, S. H. 2020. Bacteriological and Molecular Detection with Antimicrobial Resistance Pattern of Major Streptococcus spp. Isolated from Bovine Mastitis.Int.J.Curr.Microbiol.App.Sci. 9(11): 2443-2451. doi:
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