National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Dengue virus serotyping is crucial for clinical management and epidemiology of the disease. As serotype shifts and secondary infection with a heterologous DENV serotype are frequently associated with disease severity, there is a need for sustained monitoring of the circulating serotypes and enhanced surveillance operations to prevent large scale severe dengue outbreaks. Our main objective is to know the serologically laboratory confirmed dengue cases (by NS1 antigen detection ELISA) among the clinically suspected patients and to correlate the above with the prevalent serotype based on real time RT-PCR. Serum samples of clinically suspected patients with fever less than 5 days were screened for the presence of dengue NS1 antigen. Laboratory confirmed NS1 positive serum samples were sent to National Institute of Virology Pune for RT-PCR for dengue virus RNA & serotype identification. Out of 444 samples from clinically suspected dengue fever cases, NS1 antigen was positive in 71 cases (15.99%). From 71 NS1 positive samples, 48 serum samples were subjected to real time RT- PCR for serotyping of dengue virus. Dengue virus (DENV) was detected in 75% (n=36) patients out of 48 analysed samples. The predominant serotype of DENV detected in our region was DENV3. With high mortality and morbidity associated with dengue, rapid diagnosis is important. NS1 antigen detection ELISA and real time RT PCR are helpful in early diagnosis and allows rapid identification of new serotypes in endemic areas.
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