International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 2084-2090

Isolation and Identification of Escherichia Species from Faecal Sample of an Elephant and Antibiogram Assay
S.D. Audarya1*, R. Gangil1, D. Chhabra1, R. Sharda1, M. Haque2, N.Shrivastava3, S. Shuklaand U. K. Garg3
1Department of Veterinary Microbiology ,
2Department of Veterinary Parasitology,
3Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Harnyakhedi, Dr. Ambedkar Nagar, Mhow, Indore, Madhya Pradesh, India
*Corresponding author

In the present study, faecal and blood samples were collected, aseptically, from an elephant arrived to the Veterinary Clinics of the College. Immediately, samples were used for bacterial isolation. A bacterial isolate, isolated from the faecal sample of an elephant was identified at the genus level to be of Escherichiaspecies by adopting Enterobacteriaceae identification kit used for studying biochemical characteristics and carbohydrate utilization tests (Gram negativebacilli showing mainly ONPG positive, oxidase negative and nitrate reduction positive). Mueller Hinton’s agar (MHA) was used to conduct antibiogram assay by using the faecal culture. A total of 14 number of antibioticsfrom 7 different groups were utilized in the investigation namely Chloramphenicol, Ciprofloxacin, Ofloxacin, Levofloxacin, Nitrofurantoin, Norfloxacin, Tetracycline, Streptomycin, Amoxyclov, Amikacin, Ampicillin, Doxycycline hydrochloride, Penicillin G and Erythromycin. Only two antibiotics, Levofloxacin (Quinolone group) and Nitrofurantoin (Nitrofuran antibacterial) were found to be sensitive in the test.

Keywords: Elephant, Escherichia species, Antibiogram assay, Biochemical characterization, Carbohydrate utilization

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How to cite this article:

Audarya, S. D., R. Gangil, D. Chhabra, R. Sharda, M. Haque, N. Shrivastava, S. Shukla and Garg, U. K. 2020. Isolation and Identification of Escherichia Species from Faecal Sample of an Elephant and Antibiogram Assay.Int.J.Curr.Microbiol.App.Sci. 9(11): 2084-2090. doi:
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