International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 1962-1970

Comparative Microbiome Analysis of Pesticide-Contaminated and Pristine Agricultural Soils of Hot Arid Rajasthan
Anjly Pancholy* and S.K. Singh
Central Arid Zone research Institute, Jodhpur (Rajasthan) India
*Corresponding author

Comparative microbial community structure analysis of agricultural soil located in hot, western Rajasthan with long term history of chemical pesticides vis-à-vis pristine soil has been attempted using the technique of PCR-RFLP. Pesticide-contaminated (24 nos.) and pristine (10 nos.) soil samples were collected from different locations of the vegetable growing agricultural fields. Metagenomic DNA was extracted each from pooled-contaminated and pooled-pristine soils and amplified with universal 16S rRNA gene primers. Amplification products were cloned in to E. coli DH5α cells to obtain 78 and 39 16S rDNA clones from contaminated and pristine soils respectively. The clones were subjected to RFLP analysis using three restriction enzymes (AluI, AvaII and MspI) followed by phylogenetic tree construction. The studies revealed differences in microbial community structures of both types of arid, agricultural soils. We also detected the presence of pesticide-degrading naphthalene dioxygenase gene (nahA) controlling the initial step of the degradation process in contaminated soil.

Keywords: Microbiome, 16S rRNA, Pesticides, Arid soil

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How to cite this article:

Anjly Pancholy and Singh, S.K. 2020. Comparative Microbiome Analysis of Pesticide-Contaminated and Pristine Agricultural Soils of Hot Arid Rajasthan.Int.J.Curr.Microbiol.App.Sci. 9(11): 1962-1970. doi:
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