International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 1938-1945

Molecular Detection of Bulb-Associated Potyviruses in Garlic and their Field Response
Kumar Aditya1*, A.P. Bhagat1, Sangeeta Shree2 and Mohammad Ansar1
1Department of Plant Pathology,
2Department of Horticulture (Vegetable & Floriculture), Bihar Agricultural University, Sabour, Bhagalpur (Bihar), 813210, India
*Corresponding author

An experiment was designed to detect garlic bulb associated viruses through RT-PCR and to evaluate the response of genotypes against viruses under field condition. The present study was conducted at Bihar Agricultural University, Sabour, Bhagalpur, Bihar during 2019-20 to study the incidence of bulb-associated Potyviruses in garlic through molecular diagnosis. In order to confirm the presence of virus in stored garlic, RT-PCR based assay was used. The suspected leaves of genotypes were tested for the virus. Majority of genotypes were found positive with potyvirus by producing 300 bp band. Stored garlic bulbs were also tested for the virus and out of 14 genotypes, 8 were found positive with potyvirus. Under field condition, maximum genotypes showed the highest incidence at 90 days after sowing e.g. G-282 with 20.7% followed by G-1 with 15.0% disease incidence. Among all the entries, three genotypes, i.e., 499, 516 and 493 showed no any disease symptoms. The present study will be helpful to manage the crop by selecting virus-free garlic. Moreover, timely application of control strategies may be followed based on the field response of disease.

Keywords: Genotype, RT-PCR, Potyvirus, Virus-free garlic

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How to cite this article:

Kumar Aditya, A.P. Bhagat, Sangeeta Shree and Mohammad Ansar. 2020. Molecular Detection of Bulb-Associated Potyviruses in Garlic and Their Field Response.Int.J.Curr.Microbiol.App.Sci. 9(11): 1938-1945. doi:
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