International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 1884-1892

Isolation, Identification and Molecular Detection of Zoonotic Campylobacter jejuni Isolated from Mutton and Beef Samples
Sumedha Bobade*, K. Vijayarani, K. G. Tirumurugaan, A. Thangavelu and S. Vairamuthu
Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai (Tamil Nadu), India
*Corresponding author

Campylobacter species are a leading cause of food-borne disease and C. jejuni highlight the most potential public health impact of Campylobacter contamination by pathogens originating from animals or animal products.. The total of 33meat samples comprising 8 from sheep (5) and goat (3) also 25 beef samples were screened by morphological, biochemical and molecular technique. The isolates were subjected to phenotypic characterization using biochemical test and genotypic characterization. The isolates from chevon (3 out of 3) and mutton (2 out of 5) were positive for morphological and biochemical examination. The 20 (80%) beef samples were found to be positive by morphological examination and 12(48%) isolates showed biochemical reactions positive for C.jejuni. The isolates were subjected to PCR targeting hip O and MAP A genes. The result showed 66.66 % from chevon, 20% mutton and 20% isolates from beef samples were found to be positive for C.jejuni. These findings suggest that PCR should be the preferred diagnostic method for detection of Campylobacter in livestock. The good hygienic and manufacturing practices must be followed in the entire food chain to prevent the contamination of food due to microbe which can cause Campylobacteriosis among the consumers.

Keywords: CCDA, Hip O, MAP, Hippurate, C. jejuni

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How to cite this article:

Sumedha Bobade, K. Vijayarani, K.G. Tirumurugaan, A. Thangavelu and Vairamuthu, S. 2020. Isolation, Identification and Molecular Detection of Zoonotic C.jejuni Isolated from Mutton and Beef Samples.Int.J.Curr.Microbiol.App.Sci. 9(11): 1884-1892. doi:
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