National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacterial leaf blight is a disease in rice plants caused by the bacteria Xanthomonas oryzae pv oryzae L (Xoo). Biological agents controlling plant diseases that are being developed now are endophytic bacteria that can increase plant resistance to plant pathogens. This study aims to find antagonistic bacteria that are able to provide the highest resistance to the growth of pathogenic Xoo bacteria in rice plants. Antagonistic bacteria were isolated from the leaves and roots of rice plants through surface sterilization and serial dilution. Obtained 53 bacterial isolates consisting of 20 bacterial isolates from leaves and 33 bacterial isolates from roots tested hypersensitive to tobacco plants for filtering potential non-pathogenic bacteria. 29 hypersensitive negative isolates did not occur necrotic on tobacco leaves which were tested antagonist against Xoo in vitro, found 8 isolates of antagonistic bacteria derived from leaves namely AHF9LS, AHF11LS, AHF13LS, AHF3GS, AHF7GS, AHF8GS, AHF5KS, AHF12KS and 7 isolates of antagonistic bacteria from the roots namely AHE1MS, AHE9MS, AHE15MS, AHE1LS, AHE6LS, AHE11LS and AHE23KS which have the potential to inhibit Xoo growth shown by the formation of medium, strong and very strong inhibition zones. In vivo application results of 15 bacterial isolates in rice plants found bacterial isolates with very strong inhibition that is AHE1MS with the smallest average length of the lesion observed 14 days after inoculation is 0.10 cm and 28 days after inoculation is 1.27 cm. For the length of lesion in percentage of diseased leaf area AHE1MS isolate was obtained by 0.22% at 14 days after inoculation and 3.18% at 28 days after inoculation shown by AHF9LS isolates.
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