International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 1053-1058

In vitro Studies on Potentiation of Enrofloxacin by Phytochemicals
Srividya Gullapudi1*, G. S. Rao1, P. Ravikumar1, Muralidhar Metta2 and V. Ramadevi3
1Department of Veterinary pharmacology & Toxicology CVSC, Proddatur,YSR Kadapa district, Andhrapradesh-516360, India
2Department of Animal Genetics and Breeding,
3Dept of Veterinary Pathology, SriVenkateswara Veterinary University, Tirupati, India
*Corresponding author

Enrofloxacin, an antimicrobial fluoroquinolone is most commonly used against majority of gram negative bacterial and mycoplasma infections in majority of livestock. Indiscriminate usage of enrofloxacin in clinical practice leads to resistance development to this quinolne drug. Among the various pathways of resistance, efflux pump mediated drug resistance is one of the important pathways identified in the recent past. Phytochemicals namely, theobromine, glycyrrhetenic acid and glycyrrhizic acid and capsaicin were identified as efflux pump inhibitors. Phytochemicals which possess efflux pump inhibitory activity if combined with classical antimicrobial agents reduces the development of resistance and also improves their therapeutic efficacy. Interaction between enrofloxacin, and capsaicin, theobromine, glycyrrhetenic acid and glycyrrhizic acid were studied by determining MIC and MBC against E.coli, S.aureus, K.pneumoniae and P.aureginosae following CLSI guidelines. There is a significant decrease in MIC and MBC values of enrofloxacin in the presence of phytochemicals. In conclusion, Synergisitic interaction of efflux protein inhibitory phytochemicals capsaicin, theobromine, glycyrrhetenic acid and glycyrrhizic acid with enrofloxacin was noticed in minimum inhibitory concentration (MIC) and minimum bactericidal concentration.

Keywords: Enroloxacin, Capsaicin, Theobromine, Efflux pump, MIC

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How to cite this article:

Srividya Gullapudi, GS. Rao, P. Ravikumar, Muralidhar Metta and Ramadevi, V. 2020. In vitro Studies on Potentiation of Enrofloxacin by Phytochemicals.Int.J.Curr.Microbiol.App.Sci. 9(11): 1053-1058. doi:
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