International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 1040-1052

Gene Silencing in Horticultural Transgenic Plants: Mechanisms, Technologies and Applications in Horticultural Crops
S. Choudhary1, S. K. Barolia1*, R. Sharma2, R. S. R. Krishnan3, M. R. Hindalaand M. Sewda4
1ICAR-National Research Centre on Seed Spices Tabiji Ajmer, Rajasthan, India
2Biotechnology Centre, Jawaharlal Nehru Krishi Vishva Vidhalya, Jabalpur (Madhya Pradesh) India
3Jawaharlal Nehru Krishi Vishva Vidhalya, Jabalpur (MP) India
4Department of Botany, University of Rajasthan, Jaipur, India
*Corresponding author

The gene silencing in horticulture transgenic crops is too crucial to know about the internal mechanism of plant at genomic level. Horticulture involves a variety of plants and crops including fruit, vegetables, spices, and ornamental plants. Gene silencing refers to the ability of a cell to prevent the expression of a certain gene. With the cleavage or translational repression of the messenger RNA (mRNA) molecules, the genes that form them are essentially inactive. Horticultural plants have evolved a variety of gene silencing pathways in which small RNAs (20–30 nucleotides) repress the expression of genes at the transcriptional or post-transcriptional level. Gene silencing using RNA interference technology, transcriptional gene silencing, and virus-induced gene silencing has been used in horticultural crops. The presence of double-strand RNAs inside the cell lead to the production of small interfering RNAs, short hairpin RNAs, and micro-RNAs. The production of their RNAs leads to the silencing of mRNA (actual protein coating RNA). This technique is a very effective experimental tool to silence specific genes for better stress tolerance, enhance insect/pest/pathogen resistance, and improve nutritional status. This technique and mechanism can be controlled by artificial means and applied for the improvement of cultivars of a variety of horticultural crops.

Keywords: Anti-sense transgenes, DNA and RNA viruses, Flower color, modification, Parthenocarpy, Post-transcriptional gene silencing, Sense transgenes, Transgenic plants Virus resistance

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How to cite this article:

Choudhary, S., S. K.Barolia, R. Sharma, R. S. R. Krishnan, M. R. Hindala and Sewda, M. 2020. Gene Silencing in Horticultural Transgenic Plants: Mechanisms, Technologies and Applications in Horticultural Crops.Int.J.Curr.Microbiol.App.Sci. 9(11): 1040-1052. doi:
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