National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present investigation, an attempt was made to isolate endophytic fungi from rice plant (Oryza sativa L.). A total of 48 fungi were isolated from 288 segments, from 4 varieties (Gitanjali, Hiranyamayee, Khandagiri and Lalat) of rice plant (Oryza sativa L). The coloniczation frequency of the endophytic fungi were observed to be 16.87% and 16.40% in leaf and stem respectively. All the isolates were screened for GA3 production both qualitatively and quantitatively under submerged fermentation condition. Two isolates OSLST-4 and OSLL-4 exhibited maximum GA3 yield were preferred to study the effect of various physical (pH, temperature, incubation period) and nutritional (Different media, salts, carbon sources, nitrogen sources) parameters on GA3 production. It was observed that isolate OSLST-4 produced maximum amount of GA3 (96.821µg/ml) at pH-8, temperature-300C on 192hrs (8 days) of incubation under submerged fermentation in a medium containing (5%) of NaCl, (0.5%) of sucrose and (0.5%) of sodium nitrate. Whereas, the isolate OSLL-04 produced maximum amount of GA3 (78.656µg/ml) under submerged fermentation in a medium containing (3%) of NaCl, (1%) of starch and (0.3%) of ammonium chloride at pH-6, on 240hrs (10th days) of incubation period at 250C. Extracellular production of GA3 into the medium by the isolates was confirmed by TLC & FTIR analysis. Efforts are on, in our laboratory for further characterization of the isolates to exploit their potential for PGP activities for sustainable agriculture.
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