International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 527-531

Virulence Potential and Intercellular Spread of Listeria monocytogenes
Mayuri Chelkar*
Department of Veterinary Pathology, Nagpur Veterinary College, Nagpur, Maharashtra, India
*Corresponding author

Listeria monocytogenes is a gram positive, rod shaped intracellular pathogen. Its the most virulent foodborne pathogen and is responsible for human listerios is on a large scale. In this study, Listeria monocytogenes bacterium was isolated from varied sources including dairy food materials like cheese and other milk products and human infections. These isolates were cultured and allowed to proliferate in brain heart infusion broth. Isolates were screened for their proliferation and ability to infect other cells by assessing their intercellular spread and plaque formation by cell plaque assay in a murine macrophage cell line and virulence potential was determined by ANOVA methodology. Among all isolates, human origin Listeria strains demonstrated highest virulence potential.

Keywords: Listeria monocytogenes, Murine macrophage cell line, Raw 264.7, Cell to cell spread, Plaque assay

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How to cite this article:

Mayuri Chelkar 2020. Virulence Potential and Intercellular Spread of Listeria monocytogenesInt.J.Curr.Microbiol.App.Sci. 9(11): 527-531. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.