International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 176-184

SSR Markers based Molecular Assessment of Stripe Rust Resistance Genes in F3 Population of Cross DBW17 x WH1105 of Wheat (Triticum aestivum L.)
Vijeta Gupta*, Mukesh Kumar, Vikram Singh and Lakshmi Chaudhary
Dept. of Genetics & Plant Breeding, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana, India
*Corresponding author

To evaluate the presence of resistance genes parents (DBW17 and WH1105) and F3 population of cross DBW17 x WH1105 were screened for reaction to stripe rust using Modified Cobb’s Scale and using SSR markers. Considering the inheritance pattern of resistance to yellow rust a ratio of 13:3 was observed for resistant to susceptible plants. For genotyping 99 primers were used out of which 15 primers were found to be polymorphic and were dispersed over the wheat genome (AABBDD), with allele range 2-5. A total of 32 alleles were detected in polymorphic markers. The average number of SSR alleles per locus was 2.13, with a range from 2 to 4. PIC values of various SSR loci ranged from 0.44 to 0.66. The hierarchical cluster analysis revealed that the F3 population along with their parents were mainly divided into two major clusters, I and II at a similarity coefficient of 0.54. Dendrogram confirmed that two parents were highly diversed and all of the progeny lines were interspersed between the two parents. These parents can be used for developing RIL’s that could be used for QTL mapping to identify the resistance genes and chromosome location.

Keywords: F3 population, SSR markers, Resistance, Stripe rust and Wheat

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How to cite this article:

Vijeta Gupt, Mukesh Kumar, Vikram Singh and Lakshmi Chaudhary. 2020. SSR Markers based Molecular Assessment of Stripe Rust Resistance Genes in F3 Population of Cross DBW17 x WH1105 of Wheat (Triticum aestivum L.).Int.J.Curr.Microbiol.App.Sci. 9(11): 176-184. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.