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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 3876-3883
DOI: https://doi.org/10.20546/ijcmas.2020.910.446


Serotyping and Prevalence of Escherichia coli Infection in Poultry of South Gujarat based on Culture Isolation and Identification
Pratik P. Panchal1, Jignesh M. Patel1*, Priti D. Vihol1, J. K. Raval1, D. R. Patel2, Nikhil Danger3, I. H. Kalyani2 and N. M. Patel4
1Department of Veterinary Pathology, 2Department of Veterinary Microbiology, 3Department of Animal Genetics and Breeding, 4Department of Veterinary Public Health, Vanbandhu College of Veterinary Sciences and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India
*Corresponding author
Abstract:

In the present study, total 290 swab samples collected from organized poultry farms located in Navsari and Valsad districts and screened for prevalence study and different serotype of E. coli infection using culture isolation and identification. Isolation and identification resulted out of 290 swab samples 207 isolates produced lactose fermenting pink colored colonies on MacConkey agar, produced colonies with greenish metallic sheen on EMB agar and grams staining revealed typical gram-negative rods morphologically similar to E. coli. With culture isolation and identification overall prevalence was 71.38 per cent. District wise prevalence in Navsari and Valsad districts was 74.00 per cent and 65.56 per cent, respectively. Broiler birds showed higher prevalence (74.00, 148/200) rate in comparison to Layer birds (65.56, 59/90). Significantly prevalence was comparatively higher in January (90.21%) followed by September (78.78%), December (69.38%), August (51.85%) and November (40%) months. The age wise high prevalence was 82.14 per cent and 55.00 per cent in 0-30 days and 31-35 days of age group, respectively in broiler birds and 68.51 per cent and 61.11 per cent in 40-45 days and 46-50 days of age group, respectively in layer birds. As per resulted National Salmonella and E. coli Center, Kasauli,15 different serotypes out of 35 isolates, two of O8, two of O35, four of O83, three of O88, two of O119 and two of O149. High prevalence of serotype O83 (11.42%) followed by O88 (8.57), O8 (5.71), O35 (5.71), O119 (5.71) and O149 (5.71%).


Keywords: E. coli, Poultry, Isolation and Identification, Serotyping, Prevalence
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How to cite this article:

Pratik P. Panchal, Jignesh M. Patel, Priti D. Vihol, J. K. Raval, D. R. Patel, Nikhil Danger, I. H. Kalyani and Patel, N. M. 2020. Serotyping and Prevalence of Escherichia coli  Infection in Poultry of South Gujarat based on Culture Isolation and Identification.Int.J.Curr.Microbiol.App.Sci. 9(10): 3876-3883. doi: https://doi.org/10.20546/ijcmas.2020.910.446