The present research was focused in standardizing the callus induction technique and studies the behaviour of callus in secondary metabolite production in Andrographis echioides (L.) Nees. In this study, the leaf bits of Andrographis echioides responded positively for callus induction. Earliest (21.00 days) and profuse callusing was observed on MS medium supplemented with 2, 4-D (2.0 mgl^-1) + NAA (2.0 mgl^-1) + BAP (2.0
mgl^-1). The same treatment combination responded highly for callus proliferation also. Highest andrographolide content of 1.31 per cent was observed in callus grown in media supplemented with 2, 4-D (2.0 mgl^-1) + NAA (2.0 mgl^-1) + BAP (2.0 mgl^-1) combination. The alkaloid content registered an increasing trend from 45 days to 60 days of culturing.

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