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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(10): 3756-3763
DOI: https://doi.org/10.20546/ijcmas.2020.910.432


Studies on Callus Induction and in Vitro Production of Secondary Metabolite in Andrographis echioides (L.) Nees
Hemalatha1, V. Sivakumar2 and K. Rajamani3
1Agricultural Research Station, Tamil Nadu Agricultural University,
Bhavanisagar - 638 451, Tamil Nadu, India
2 Coconut Research Station, Tamil Nadu Agricultural University,
Aliyarnagar - 642 101, Tamil Nadu, India
3Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore - 641 003, India
*Corresponding author
Abstract:

The present research was focused in standardizing the callus induction technique and studies the behaviour of callus in secondary metabolite production in Andrographis echioides (L.) Nees. In this study, the leaf bits of Andrographis echioides responded positively for callus induction. Earliest (21.00 days) and profuse callusing was observed on MS medium supplemented with 2, 4-D (2.0 mgl-1) + NAA (2.0 mgl-1) + BAP (2.0
mgl-1). The same treatment combination responded highly for callus proliferation also. Highest andrographolide content of 1.31 per cent was observed in callus grown in media supplemented with 2, 4-D (2.0 mgl-1) + NAA (2.0 mgl-1) + BAP (2.0 mgl-1) combination. The alkaloid content registered an increasing trend from 45 days to 60 days of culturing.


Keywords: MS medium, Explant, Callus, Proliferation, Andrographolide
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How to cite this article:

Hemalatha, P., V. Sivakumar and Rajamani, K. 2020 Studies on Callus Induction and in Vitro Production of Secondary Metabolite in Andrographis echioides (L.) NeesInt.J.Curr.Microbiol.App.Sci. 9(10): 3756-3763. doi: https://doi.org/10.20546/ijcmas.2020.910.432