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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 114-120
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.016


Characterization and Antibiotic Susceptibility Pattern of Coagulase Negative Staphylococci with Special Reference to Methicillin Resistance
Rashmi M Karigoudar1* and Mahantesh B Nagamoti2
1Department of Microbiology, BLDE University’s Shri BM Patil Medical College and RC Solapur Road, Vijayapur-586103, Karnataka, India
2Department of Microbiology, KLE University’s, JN Medical College, Belagavi- 590010, Karnataka, India
*Corresponding author
Abstract:

Coagulase negative staphylococci (CONS) being the most common inhabitants of skin and mucous membranes are known to be the commonest opportunistic pathogens causing infections among the immune compromised hosts as well as the patients with medical devices such catheters, prosthetic heart valves and orthopaedic prosthesis. In recent years, an increase in the number of methicillin resistant coagulase negative Staphylococci (MRCONS) strains has become a serious public health problem, as the resistance for this antibiotic implies resistance to almost all the beta–lactum antibiotics. To assess the antibiotic susceptibility pattern of coagulase negative staphylococci with special reference to methicillin resistance. A total of 120 strains of CONS isolated in pure culture from various clinical samples were subjected to speciation and antibiotic susceptibility pattern with special reference to methicillin. In addition these isolates were also tested for slime production and beta-lactamase production. Antimicrobial susceptibility testing was performed by Kirby Bauer disc diffusion method. Out of 120 CONS isolates, 65.5% were S. epidermidis, 15% S. saprophyticus, 10.83% S haemolyticus, 3.33% S. Scuiri, 1.67% S hominis, 1.67% S Xylosus, 1.67% S Cohnii, 0.83% S Simulans. Majority of the isolates were from pus followed by blood and urine. Among the various organisms 55.83% were slime producing organisms. 37.5% isolates showed Beta-lactamase production and 40% of the species showed resistant to methicillin antibiotic. Most of the species had also showed resistance to penicillin, ampicillin and gentamicin. The increasing recognition of CONS & emergence of drug resistance demonstrates the need for characterization and determination of antibiotic susceptibility of CONS with special reference to methicillin has become warranted.


Keywords: Coagulase negative, Staphylococcus, Methicillin, Antibiotic susceptibility, Antibiotic resistance.
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How to cite this article:

Rashmi Karigoudar, M., Mahantesh Nagamoti, B. 2016. Characterization and Antibiotic Susceptibility Pattern of Coagulase Negative Staphylococci with Special Reference to Methicillin Resistance.Int.J.Curr.Microbiol.App.Sci. 5(3): 114-120. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.016